针对HER2的CPP-PTEN-THP化学蛋白的抗癌活性
Elizeth Pioquinto-Avila1, Aldo O González-Cruz1, Jorge Solís-Estrada1
1Laboratorio de Farmacología Molecular y Modelos Biológicos, Facultad de Ciencias Químicas, Universidad Autonoma de Nuevo Leon, UANL, Nuevo León, México.
Anticancer research
|May 31, 2024
概括
基于瘤抑制剂蛋白酸酶和素同源 (PTEN) 的新型仿制蛋白显示出针对HER2阳性乳腺癌细胞的特定抗癌作用. 这些工程 PTEN 蛋白质证明了向的输送和抑制癌细胞生长.
科学领域:
- 生物技术是生物技术.
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 蛋白酸酶和素同源 (PTEN) 是一个关键的瘤抑制剂.
- PTEN 缺乏与各种癌症有关,是治疗开发的目标.
- 目前癌症药物输送的局限性包括瘤透率低和选择性低.
研究的目的:
- 为针对性治疗设计基于PTEN的化学蛋白质 (CPP-PTEN-THP).
- 评估这些蛋白质对人类表皮生长因子受体2 (HER2) 阳性乳腺癌的疗效.
- 评估开发的蛋白质的HER2特异性抗癌作用和传递能力.
主要方法:
- 使用pCEFL-EGFP矢量构建图形蛋白TAT-PTEN-LTV和KLA-PTEN-LTV的方法.
- 在HEK-293T细胞中通过西部涂抹证实了蛋白质表达.
- 使用HCC-1954 (HER2阳性) 和MCF-7细胞系的非接触共培的细胞毒性测定.
主要成果:
- 重组PTEN仿制蛋白的成功表达,与内源PTEN相比,其水平较高.
- 通过KLA-PTEN-LTV (25.95±0.9%) 和TAT-PTEN-LTV (12.25±1.29%) 优先抑制HCC-1954细胞生长.
- 对接分析表明,LTV部分和HER2之间存在特定的相互作用,其中介于Pro,Trp和轮胎残留物.
结论:
- 开发的基于PTEN的化学蛋白质表现出HER2特异性抗癌活性.
- 这些工程蛋白显示出针对HER2阳性乳腺癌的向治疗的潜力.
- 这些发现支持了仿真PTEN蛋白对癌症治疗的治疗潜力.
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