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非侵入性物理等离子调节巨细胞极化:瘤微环境重塑的潜在策略

Yuequn Niu¹, Sarah Förster¹, Steffen Badendieck²

¹Department of Pathology, University Hospital Bonn, Bonn, Germany.

Anticancer research

|May 31, 2024

概括

非侵入性物理等离子体 (NIPP) 安全诱导氧化应激,促进M1巨细胞的两极分化. 这表明NIPP是NIPP.

科学领域:

生物医学工程生物医学工程
癌症研究癌症研究
免疫学免疫学免疫学

背景情况:

非侵入性物理等离子体 (NIPP) 在癌症治疗中显示出治疗潜力.
关于NIPP对巨细胞两极分化的影响存在相互矛盾的数据.
与瘤相关的巨细胞 (TAMs) 极大地影响了癌症的进展.

研究的目的:

为了研究NIPP对巨细胞两极分化的影响.
确定NIPP在调节瘤微环境 (TME) 中的作用.

主要方法:

在THP-1细胞上使用便携式NIPP设备 (血护理).
评估了NIPP对培养基温度,pH和氧化应激的影响.
通过 qPCR 评估 THP-1 细胞形态,活力,增殖和 M1/M2 标志物表达.

主要成果:

NIPP治疗没有改变温度或pH值,但随着时间的推移,过氧化增加了.
细胞活力和增殖没有受到NIPP的影响,长达6分钟.
在单细胞和巨细胞中,NIPP显著上调M1标记物 (TNF-α,IL-6) 和下调M2标记物 (CD206),促进M1极化.

结论:

NIPP是一种安全有效的氧化应激诱导剂.
通过促进M1两极分化,NIPP展示了TAM调节的潜力.
在癌症治疗中,NIPP可能为TME调制提供一种新的方法.

关键词:

非侵入性的物理等离子体.这就是THP-1的特点.寒冷的大气等离子体是什么?冰冷的等离子体是什么?免疫学免疫学免疫学巨细胞的两极分化组织可容忍的血.

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