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Protein Kinases and Phosphatases02:54

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
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The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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阿尔法-同核素聚合物具有对酸酶的耐药性.

S G Choi1, T Tittle1, D Garcia-Prada1

  • 1Department of Neurological Sciences, Rush University Medical Center, Chicago, IL, USA.

Acta neuropathologica communications
|June 1, 2024
PubMed
概括

酸化α-synuclein (αsyn) 在血清129 (PSER129) 发生在健康的大脑中. 牛犊肠酸酶 (CIAP) 处理在哺乳动物组织中区分内源和聚合的PSER129.

关键词:
帕金森病的发病因子后翻译修改后的修改.验尸后的时间间隔.蛋白质聚合蛋白质的聚合.

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科学领域:

  • 神经科学是一个神经科学.
  • 生物化学 生物化学
  • 病理学 病理学 病理学

背景情况:

  • 阿尔法-同核素 (αsyn) 聚合是同核素病变的核心.
  • 血清129 (PSER129) 的酸化是病理性αsyn的标志,但也发生在健康的神经元中.
  • 将内源PSER129与聚合PSER129区分开来,对于了解疾病机制至关重要.

研究的目的:

  • 研究检测和区分内源性和聚合PSER129池的条件.
  • 确定固定和麻醉条件对PSER129检测的影响.
  • 评估小牛肠酸酶 (CIAP) 在区分PSER129池中的实用性.

主要方法:

  • 检查了野生类型的老鼠大脑,随着死亡后间隔的变化.
  • 评估了麻醉剂 (基他胺,西拉) 对PSER129水平的影响.
  • 利用现场CIAP处理来评估内源和聚合PSER129.29的酸酶敏感性.
  • 与蛋白酶K (PK) 抗性αsyn.syn.相关的CIAP耐药性.

主要成果:

  • 延迟的输液固定显著降低了内源的PSER129检测.
  • 麻醉剂没有显著影响内源PSER129水平.
  • 内源PSER129被CIAP选择性地去化,而聚合形式则具有耐药性.
  • CIAP电阻与PK电阻相关,表明聚合.
  • 通过CIAP的预处理,可以对种子合聚合物进行特定检测.

结论:

  • 阿尔法-同核素聚合物对酸酶处理具有抗性.
  • CIAP预处理提高了在保存良好的样本中检测聚合PSER129的特异性.
  • 这种方法有助于理解PSER129在同核蛋白病变中的积累,并区分与疾病相关的形式.