Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Transcriptomic and metabolomic analysis of autumn leaf color change in <i>Fraxinus angustifolia</i>.

PeerJ·2023
Same author

Chitosan-Linked Dual-Sulfonate COF Nanosheet Proton Exchange Membrane with High Robustness and Conductivity.

Small (Weinheim an der Bergstrasse, Germany)·2023
Same author

Pulmonary Artery Denervation Inhibits Left Stellate Ganglion Stimulation-Induced Ventricular Arrhythmias Originating From the RVOT.

JACC. Clinical electrophysiology·2023
Same author

High-Temperature-Induced Pore System Evolution of Immature Shale with Different Total Organic Carbon Contents.

ACS omega·2023
Same author

Frequency Domain Filtering Method for SSVEP-EEG Preprocessing.

IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society·2023
Same author

CCL12 induces trabecular bone loss by stimulating RANKL production in BMSCs during acute lung injury.

Experimental & molecular medicine·2023

相关实验视频

Updated: Jun 24, 2025

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

68.6K

哺乳动物表转录组的定量绘制.

Bo He1, Yuting Chen2, Chengqi Yi3

  • 1Peking University Chengdu Academy for Advanced Interdisciplinary Biotechnologies, Chengdu, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.

Current opinion in genetics & development
|June 1, 2024
PubMed
概括

本综述探讨了检测RNA修饰的先进方法,这对于理解它们的作用至关重要. 它强调了为表表转录学研究提供基准分辨率和定量数据的技术.

更多相关视频

Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes
08:18

Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes

Published on: April 24, 2019

10.7K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.3K

相关实验视频

Last Updated: Jun 24, 2025

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

68.6K
Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes
08:18

Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes

Published on: April 24, 2019

10.7K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.3K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生物化学

背景情况:

  • 表体转录组涉及动态RNA修改影响RNA功能.
  • 已知有170多种RNA化学修饰,需要先进的检测工具.
  • 基准分辨率和定量数据对于研究RNA修饰调节和功能至关重要.

研究的目的:

  • 审查检测RNA修饰的方法,以基本分辨率和定量准确度.
  • 讨论在定量RNA修饰检测当前的挑战和未来的前景.

主要方法:

  • 现有的定量RNA修饰检测方法的概述.
  • 专注于下一代测序和纳米孔直接RNA测序.
  • 分析提供基本分辨率和石化信息的技术.

主要成果:

  • 确定了两个主要的定量RNA修饰检测类别:下一代测序和纳米孔直接RNA测序.
  • 强调了基础分辨率和定量数据对于理解RNA修饰的重要性.
  • 突出了该领域的挑战和未来方向.

结论:

  • 创新的检测方法对于进步表皮转录组学至关重要.
  • 定量和基础分辨率技术是解锁RNA修饰功能的关键.
  • 需要继续开发以克服RNA修饰检测当前的挑战.