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建立人类接种疫苗后的SARS-CoV-2标准参考血清

Jinhua Xiang¹, Louis Katz², Patricia L Winokur³

¹Iowa City Veterans Administration Healthcare System, 601 Highway 6, Iowa City, IA 52246, USA; The University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA.

Journal of immunological methods

|June 1, 2024

概括

研究人员从接种了SARS-CoV-2 (COVID-19) 疫苗的个人中创建了标准化血清参考试剂,以评估疫苗对不断变化的变种的有效性. 这些试剂能够在实验室环境中对中和抗体进行一致的评估.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
疫苗学疫苗学疫苗学

背景情况:

COVID-19大流行突显了SARS-CoV-2的快速传播和演变.
疫苗诱导的免疫力减弱和新出现的变种减少了保护.
需要标准化方法来评估对SARS-CoV-2变种的中和抗体反应.

研究的目的:

从各种SARS-CoV-2疫苗接种者的标准化血清参考试剂中生成和描述.
为了使疫苗引起的抗体中和 in vitro 的实验室间一致的评估.
为评估针对SARS-CoV-2变种的疫苗有效性提供一个工具.

主要方法:

从接受初级和增强型SARS-CoV-2疫苗 (mRNA,Ad26,NVX-CoV2373) 的个体收集和聚合血清.
查血清以检测没有先前的感染标志物 (核体抗体).
标志性血清抗体用于尖端蛋白结合,ACE2结合竞争和伪型的lentivirus中和.

主要成果:

从各种SARS-CoV-2疫苗平台的接收者中生成了特征血清池.
建立了针对抗体结合和中和能力的标准化试验.
通过BEI资源向研究界提供这些参考试剂.

结论:

开发的血清试剂允许对SARS-CoV-2中和进行标准化,可比的评估.
这些试剂对于评估疫苗诱导的抗体对当前和新出现的SARS-CoV-2变体的疗效至关重要.
促进对疫苗有效性的研究和下一代疫苗的开发.

关键词:

抗体是一种抗体.在 COVID-19 疫情中,参考试剂是指参考试剂的使用情况.这就是SARS-CoV-2病毒.疫苗疫苗疫苗疫苗

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