超声波辅助从古吉果中提取生物活性化合物:优化,生物活性和肠道透性评估
Filipa Teixeira1, Ana Margarida Silva2, Stefania Sut3
1REQUIMTE/LAQV, ISEP, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal; Chemical and Biomolecular Sciences, School of Health, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida 400, 4200-072 Porto, Portugal.
果提取物使用超声波辅助提取优化,显示显著的抗氧化能力. 像glu-lycibarbarspermidine F这样的关键化合物证明了肠道透,这表明真正的健康益处.
科学领域:
- 药理学 药理学是指药理学的学科.
- 营养学科学 营养学科学
- 食品化学 食品化学
背景情况:
- 草野蛮L. (戈吉果) 含有丰富的生物活性化合物,具有潜在的健康益处.
- 了解这些化合物的肠道透对于预测它们的体内功效至关重要.
- 目前关于古吉的生物活性化合物吸收和生物利用性的知识是有限的.
研究的目的:
- 优化使用响应表面方法 (RSM) 的果生物活性化合物的超声波辅助提取 (UAE).
- 为了建立从优化的古吉果提取物中获得关键化合物的肠道透.
- 为了将植物化学成分与观察到的生物活动相关联.
主要方法:
- 超声波辅助提取 (UAE) 通过响应表面方法 (RSM) 进行了优化.
- 液体染色学/二极管阵列检测-喷射电离-质谱学 (LC/DAD-ESI-MS) 用于植物化学分析.
- 在体外肠道共同培养模型来评估化合物透.
主要成果:
- 确定了阿联最佳条件:8.75%的固体:液体比率,56.21分钟,59.05W/m2强度.
- 优化的提取物表现出显著的抗氧化能力.
- LC/DAD-ESI-MS确定了各种化合物,包括葡萄糖-lycibarbarspermidine F,2-glu-kukoamine, rutin 和 3,5-dicaffeoylquinic 酸.
- 格鲁-利西巴巴斯米丁F (同位素2),3,5-迪卡菲奥尔基因酸,和异胺-3-O-鲁丁oside显示显著的肠道透.
结论:
- 优化阿联有效地从古吉果中提取生物活性化合物.
- 一些已识别的化合物具有穿透肠道屏障的能力.
- 这些发现支持在营养品中使用古吉果,并突出了特定生物活性化合物的潜在健康促进作用.
更多相关视频
07:05Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques
Published on: August 23, 2024
06:00Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development
Published on: March 17, 2023
相关概念视频
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Factors Influencing Bioavailability: First-Pass Elimination
Factors Influencing Drug Absorption: Physicochemical Parameters
Enhanced drug absorption can be achieved by reducing particle sizes and increasing surface areas, thereby facilitating...
Drug Absorption: Factors Affecting GI Absorption
