使用离子液体作为乳化剂提高大麻二醇的生物可访问性,以产生纳米系统:结构的表征,细胞毒性评估和体外消化
Renata Vardanega1, Fernanda L Lüdtke1, Luís Loureiro1
1Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal; LABBELS - Associate Laboratory, Guimarães, Portugal.
Food research international (Ottawa, Ont.)
|June 1, 2024
概括
油酸盐是一种生物相容的离子液体 (IL),有效地稳定纳米乳液和纳米结构脂质载体中的大麻 (CBD). 这些基于IL的纳米系统提高了CBD的生物可访问性,并降低了其细胞毒性.
科学领域:
- 制药纳米技术 制药纳米技术
- 生物相容材料科学 生物相容材料科学
- 大麻素治疗药物 大麻素治疗药物
背景情况:
- Cannabidiol (CBD) 的生物可访问性通常受到其水溶性较差的限制.
- 开发稳定有效的输送系统对于增强CBD的治疗潜力至关重要.
- 离子液体 (ILs) 为制定先进的药物输送系统提供了新的特性.
研究的目的:
- 研究一种生物相容的离子液体 (胆烯酸盐) 的乳化能力,以创建含有CBD的脂质纳米系统.
- 评估通过纳米乳液 (NE-IL) 和纳米结构脂质载体 (NLC-IL) 输送的CBD的稳定性,封装效率和生物可访问性.
- 评估这些纳米系统对CBD体外细胞毒性的影响.
主要方法:
- 使用1%和2%度的胆油酸盐制备含有CBD的纳米乳液 (NE-IL) 和纳米结构脂质载体 (NLC-IL).
- 评估纳米系统的稳定性,捕获效率和28天的CBD保留时间.
- 在体外消化研究评估CBD的稳定性和生物可访问性.
- 细胞毒性测试以确定纳米系统配方对CBD细胞毒性的影响.
主要成果:
- 1%的胆酸盐度足以产生稳定的NE-IL和NLC-IL,捕获效率高于99%和100%的CBD保留28天.
- 在体外消化过程中,NLC-IL证明了CBD的优越稳定性.
- NE-IL显著改善了CBD的生物可访问性,这归因于脂质矩阵组成.
- 在NE-IL和NLC-IL中捕获CBD减少了其观察到的细胞毒性.
结论:
- 酸是一种有前途的生物相容剂,可用于制造稳定有效的CBD载脂纳米系统.
- 在NE-IL和NLC-IL之间做出选择可以量身定制,以优化CBD稳定性或生物可访问性.
- 这些基于IL的纳米系统代表了增强CBD输送和减轻其潜在毒性的可行策略.
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