通过DPCfam集群对统一的人类胃肠蛋白质组的蛋白质家族注释
Federico Barone1,2, Elena Tea Russo1, Edith Natalia Villegas Garcia1,2
1Area Science Park, Padriciano, 99, 34149, Trieste, Italy.
Scientific data
|June 1, 2024
概括
本研究介绍了DPCfam-UHGP50数据集,对人类肠道微生物群中的蛋白质家族进行分类. 该资源增强了元基因组数据的注释,有助于未来的肠道微生物组研究.
科学领域:
- 转基因组学是指转基因组学.
- 蛋白质组学是指蛋白质组学.
- 生物信息学是一种生物信息学.
背景情况:
- 大规模并行测序推动了蛋白质序列数据的指数增长,特别是来自元基因组项目.
- 统一的人类胃肠蛋白质组 (UHGP) 是一个关键的元基因组数据集,但有限的注释阻碍了它的实用性.
- 准确的蛋白质家族分类对于理解生物功能和结构至关重要.
研究的目的:
- 为了改善结构和功能注释,在UHGP数据集中对蛋白质家族进行分类.
- 创建DPCfam-UHGP50数据集,这是人类肠道蛋白质组分析的全面资源.
- 为了提高生物医学和生物研究的元基因组数据的可用性.
主要方法:
- 利用DPCfam集群,一个无监督的管道,以基于域架构的组序列.
- 为DPCfam-UHGP50数据集生成了10778个假定蛋白质家族.
- 开发了一个符合FAIR的数据库,具有可搜索的Web服务器和Zenodo存储库.
主要成果:
- 与Pfam.fam相比,DPCfam-UHGP50数据集在蛋白质和残留水平上提供了增强的蛋白质家族覆盖范围.
- 成功地将大量的UHGP序列分类为假定蛋白质家族.
- 为DPCfam-UHGP50数据集建立了一个可访问和可搜索的数据库.
结论:
- DPCfam-UHGP50数据集显著改善了人类肠道蛋白质组的注释.
- 这一资源有望促进人类肠道转基因组学的新发现.
- 发布符合FAIR标准的数据库确保了广泛的可访问性,并促进了进一步的研究.
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