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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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相关实验视频

Updated: Apr 10, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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基于空间角度双重复杂化全息的单曝光多波长光学衍射断层扫描.

Hong-Yi Huang, Qing-Yang Yue, Yang Yang

    Optics letters
    |June 2, 2024
    PubMed
    概括

    我们开发了一种新的全息系统,用于快速,单次曝光的多波长光学衍射断层扫描 (ODT) 成像. 这种方法可以在没有扫描的情况下重建3D折射率断层扫描,从而推进生物和材料科学成像.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 生物医学成像技术 生物医学成像技术
    • 断层扫描 (Tomography) 是一个专业的技术.

    背景情况:

    • 多波长光衍射断层扫描 (ODT) 为生物标本提供了丰富的对比度.
    • 现有的ODT方法通常需要扫描或多次曝光,限制成像速度和适用性.
    • 全息技术提供高分辨率成像,但将它们与多波长ODT集成是具有挑战性的.

    研究的目的:

    • 开发用于多波长光衍射断层扫描 (ODT) 成像的单次曝光系统.
    • 为了使样本的快速3D折射率重建成为可能.
    • 为了克服当前ODT系统中扫描机制和多次曝光的局限性.

    主要方法:

    • 设计了一个空间角度双重复合全息记录系统.
    • 该系统结合了用于多波长全息的角度分割复合和用于ODT的微镜阵列多角度照明.
    • 开发了一种新的数据处理方法,用于从单个全息图中复制断层图像.

    主要成果:

    • 该系统成功地在单个摄像头曝光中记录了所有衍射断层扫描信息.
    • 多波长3D折射率断层扫描仪从单个复杂化全息图中重建.
    • 使用聚钢珠和活生生的C. elegans虫来证明可行性.

    更多相关视频

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
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    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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    相关实验视频

    Last Updated: Apr 10, 2026

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

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    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
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    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

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    结论:

    • 拟议的空间角度双重复杂化系统可以实现高效的单曝光多波长ODT成像.
    • 这种技术显著加快了3D折射率信息的获取速度.
    • 该系统对生物样品的无标签,高分辨率成像具有前景.