基于4EBP1的mTORC1活细胞传感器TORSEL揭示了由组织素脱乙酶抑制剂对营养感应的向
Canrong Li1, Yuguo Yi1, Yingyi Ouyang1
1School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, China.
Cell & bioscience
|June 2, 2024
概括
科学家们开发了TORSEL,这是一种新型传感器,可以实时监测拉巴胺素复合体1 (mTORC1) 活性的机械标. 该工具有助于发现新的mTORC1抑制剂,用于癌症和神经退行等疾病.
科学领域:
- 蜂信号传输是如何进行的
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 哺乳动物或机械向的拉巴胺复合物1 (mTORC1) 是一个关键的调节器,与癌症,糖尿病,衰老和神经退行有关.
- 对于有效的工具来监测活细胞和组织中的mTORC1抑制,存在着至关重要的需求.
研究的目的:
- 开发一种基因编码的传感器,用于实时监测mTORC1活动.
- 为了验证传感器在检测各种形式的mTORC1抑制方面的有效性.
- 为了利用传感器选和识别新型mTORC1抑制剂.
主要方法:
- 基于4EBP1脱和eIF4E相互作用的基因编码传感器TORSEL的开发.
- 使用TORSEL对活细胞和组织进行基于成像的视觉查.
- 对TORSEL对mTORC1抑制的特异性的药理学和遗传学验证.
主要成果:
- 在mTORC1抑制时,TORSEL表现出明显的光转变,从扩散到点.
- 传感器准确地检测到mTORC1信号传递的生理,药理和遗传抑制.
- 托尔塞尔促进了对选择性向营养感应通路以抑制mTORC1.1.的基因脱乙酶抑制剂的鉴定.
结论:
- 托塞尔作为一个独特而高效的活细胞传感器来检测mTORC1活动.
- 鉴定出包括泛比诺斯塔特在内的基因组脱乙酶抑制剂是通过氨基酸感应通路准mTORC1信号的药物.
更多相关视频
08:04Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
18.9K
11:16Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
Published on: July 12, 2014
37.0K
相关概念视频
01:22PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K