关于微流体单细胞培养和对中国仓鼠卵巢悬浮细胞系活细胞成像的协议
Julian Schmitz1, Boris Yermakov2, Alexander Grünberger2
1Multiscale Bioengineering, Faculty of Technology, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.
STAR protocols
|June 2, 2024
概括
这项研究详细介绍了一项强大的协议,用于微流体单细胞培养 (MSCC) 的中国仓鼠卵巢 (CHO) 细胞. 它确保可靠和可重复的结果,用于工业应用.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 生物工艺工程 生物工艺工程
背景情况:
- 微流体单细胞培养 (MSCC) 能够详细研究细胞行为.
- 中国仓鼠卵巢 (CHO) 细胞对于生物制药生产至关重要.
- 需要对CHO悬浮细胞的MSCC制定标准化协议.
研究的目的:
- 为工业相关的CHO悬浮细胞系的MSCC提供可靠和可重复的协议.
- 优化关键步骤,以成功培养CHO细胞的单细胞培养.
- 促进MSCC在生物过程开发和细胞系表征中的应用.
主要方法:
- 开发和优化微流体单细胞培养协议.
- 专注于培养前管理和介质准备.
- 设备注射和恒定介质 perfusion 的详细程序.
主要成果:
- 建立了一个可复制的CHO悬浮细胞MSCC的综合性协议.
- 确定了成功的种子列车,介质制备和 perfusion 的关键参数.
- 该协议支持可靠的CHO细胞单细胞分析.
结论:
- 提出的协议使可靠和可重复的微流体单细胞培养可靠和可重复的CHO悬浮细胞.
- 这种方法对于在单细胞水平上研究CHO细胞行为是有价值的.
- 该协议可以推进生物过程开发和涉及CHO细胞系的研究.
更多相关视频
07:05Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
Published on: September 27, 2019
6.0K
08:43Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
Published on: August 9, 2024
907
相关概念视频
Higher Mental Functions of the Brain: Language
Language is a system of communication that allows the expression of thoughts, ideas, and feelings. The brain processes language in both hemispheres.
Language formation and comprehension take place in the dominant hemisphere. The dominant hemisphere is responsible for understanding the meaning of spoken, written, or sign language, as well as the ability to communicate. For most people, the left hemisphere is the dominant one. The right hemisphere, then, gives tone and emotional context to the...
Language formation and comprehension take place in the dominant hemisphere. The dominant hemisphere is responsible for understanding the meaning of spoken, written, or sign language, as well as the ability to communicate. For most people, the left hemisphere is the dominant one. The right hemisphere, then, gives tone and emotional context to the...
Cerebral Hemispheres
The human brain, a complex organ, is functionally divided into two cerebral hemispheres—left and right. These hemispheres are interconnected by a structure of paramount importance, the corpus callosum. This substantial bundle of neural fibers is not just a bridge between the hemispheres but a crucial element for the brain's comprehensive functioning. It enables efficient communication between the two hemispheres, allowing each side of the brain to control and receive sensory and motor...
Lateralization
Brain lateralization refers to the division of mental processes and functions between the two hemispheres of the brain, a phenomenon that optimizes neural efficiency and underpins complex abilities in humans. This specialization allows each hemisphere to perform tasks where it has a comparative advantage, facilitating more refined cognitive capabilities across different domains.
Language and Cognition
Language serves as a bridge between ideas and communication, influencing how individuals perceive and interact with the world. Psychologists have long debated whether language shapes thought or vice versa. This discussion gained grip with Edward Sapir and Benjamin Lee Whorf in the 1940s, who proposed that language determines thought, a concept known as linguistic determinism. They suggested that the vocabulary and structure of a language influence how its speakers think and perceive reality.
Learning Disabilities
Learning disabilities are cognitive disorders caused by neurological impairments that affect cognitive functions like language and reading, without indicating overall intellectual or developmental challenges. These disabilities differ from global intellectual or developmental disabilities as they are limited to distinct cognitive functions. Common learning disabilities include dysgraphia, dyslexia, and dyscalculia, each of which impacts unique aspects of learning.
Dyslexia
Dyslexia is a...
Dyslexia
Dyslexia is a...
