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实现了可访问性和低成本的光学投射断层扫描 (OPTImAL)

C Darling¹, S Kumar^1,2, Y Alexandrov^1,2

¹Physics Department, Imperial College London , London SW7 2AZ, UK.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences

|June 3, 2024

概括

我们开发了一种负担得起的,开源的光学投影断层扫描 (OPT) 系统,用于3D成像. 这种可访问的光OPT使研究人员能够以减少光漂白和光毒性对样品进行成像.

科学领域:

生物医学成像技术生物医学成像技术
显微镜的使用方法
光学物理学的光学物理学

背景情况:

光学投影断层扫描 (OPT) 是一种用于 mesoscopic 样品的 3D 成像技术.
现有的OPT系统可能昂贵而复杂,限制了研究的可访问性.

研究的目的:

展示一个可访问的,低成本的,开源的光OPT的实现.
为了使OPT在生物研究中得到更广泛的采用.

主要方法:

开发了模块化OPT硬件,使用LED和CMOS摄像头等经济实惠的组件.
实现了灵活的软件,支持压力传感,用于快速成像.
探索焦点扫描OPT与风扇光束重建以获得更高分辨率.

主要成果:

展示了一个研究级的模块化OPT系统,成本降低.
使用压缩传感实现了低光漂白/光毒性快速成像.
展示了使用焦点扫描进行更高分辨率成像的潜力.

结论:

开发的开源OPT系统增强了3D中视镜成像的可访问性.
这种负担得起的平台可提供先进的成像技术,减少样品损伤.
通过开放的硬件和软件,促进显微镜中的可重复性研究.

关键词:

显微镜显微镜是指使用显微镜.开放式硬件开放式硬件这是一个开源的开源软件.光学投射断层扫描仪

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