循环时钟神经元使用活动调节的基因表达来实现结构性可塑性
bioRxiv : the preprint server for biology
|June 3, 2024
概括
果的昼夜神经元在黎明时扩展投影,由活动调节基因控制,如Hr38和Sr. 这些基因将神经元活动与结构性可塑性联系起来,这对日常节奏至关重要.
科学领域:
- 神经科学是一个神经科学.
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- * *Drosophila*小腹侧侧神经元 (s-LNvs) 呈现出显著的结构性可塑性,在黎明时延伸并在黄昏时收缩的投射.
- * 在s-LNv结构中,这种每日节奏受到分子钟和神经元刺激性的影响.
- * 了解这种可塑性的分子基础是解读昼夜调节的关键.
研究的目的:
- * 调查活动调节基因是否调解Drosophila* s-LNvs.中的结构性可塑性.
- * 确定涉及将神经元活动与s-LNv投射动态联系的特定基因.
- *阐明连接昼夜时钟,刺激性和结构变化的分子途径.
主要方法:
- * 在 *Drosophila* s-LNvs. 中对转录因子表达 (Hr38,Sr) 的基因操纵.
- *体内成像和分析s-LNv投射形态.
- *基因淘汰实验,以评估Hr38和Sr对于可塑性的必要性.
- *下游基因标的分析,包括*sif*.
主要成果:
- *活动调节转录因子Hr38或Sr的过度表达迅速诱导了s-LNv投影扩张.
- * *Hr38*或*sr*的淘汰阻止了黎明特定的s-LNv投影扩张.
- *Hr38被证明可以直接诱导*sif*的转录,这是一种编码Rac1 GEF的基因,对可塑性节奏至关重要.
结论:
- 果的分子时钟调节神经元的刺激能力.
- *这种刺激性改变了夫妇的活性调节基因表达程序,以控制日常结构可塑性.
- * Hr38和Sr在连接神经元活动与昼夜结构节律的途径中起到关键的调解作用.
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