Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Eight-qubit operation of a 300 mm SiMOS foundry-fabricated device.

Nature communications·2026
Same author

The Utilisation, Knowledge and Opinions Regarding Static Computer-Assisted Implant Surgery (s-CAIS) Among Australian and New Zealand Dental Practitioners: A Survey.

International journal of dentistry·2026
Same author

IFNγ-producing iNKTs restrict a live-attenuated chlamydia oral vaccine in the large intestine.

Frontiers in immunology·2026
Same author

Distinguishing Photoacoustic and Photothermal Neuron Stimulation Through Quantitative Mapping Spatiotemporal Field Evolution.

bioRxiv : the preprint server for biology·2026
Same author

Chlamydial histone homologs control developmental fitness in the next infection cycle.

mSphere·2026
Same author

Transparent Adhesive Labels for Mohs Micrographic Surgery Mapping Training.

Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]·2026

相关实验视频

Updated: Jun 24, 2025

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

13.7K

分析超广泛转录基因激活的Chlamydia的RNA-Seq数据:其他系统的挑战,解决方案和影响.

Danny Wan1, Andrew Cheng1, Yuxuan Wang1

  • 1Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA.

bioRxiv : the preprint server for biology
|June 3, 2024
PubMed
概括

标准的RNA测序分析与克拉米迪亚转录组相斗争. 修订后的正常化方法准确地捕获基因表达变化,在早期感染中识别了700多个升调的基因.

更多相关视频

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.2K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.5K

相关实验视频

Last Updated: Jun 24, 2025

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

13.7K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.2K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.5K

科学领域:

  • 微生物学 微生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 标准的RNA测序 (RNA-Seq) 分析管道,如DESeq2和edgeR,面临着像Chlamydia spp.这样的有义务细胞内细菌的转录组的限制.
  • 这些局限性源于数据集中未满足的假设,具有显著的转录组激活和最小的抑制,在早期细菌感染阶段很常见.

研究的目的:

  • 用标准RNA-Seq差异表达方法来解决分析克拉米迪亚早期转录组的挑战.
  • 开发和验证修订的规范化策略,以准确评估甲状腺菌基因表达动态.

主要方法:

  • 通过结合细菌和宿主RNA-Seq读数来实施修订后的规范化技术.
  • 在RNA-Seq数据分析中为总测序深度进行调整.
  • 使用定量逆转录PCR (qRT-PCR) 验证发现.

主要成果:

  • 单一的克拉米迪亚读数的标准分析确定了大约300个上调和300个下调的基因,误导了实际的RNA-Seq趋势.
  • 经过修订的正常化方法,包括宿主读取或根据测序深度进行调整,检测到700多个上调基因和不到30个下调基因.
  • qRT-PCR证实了调整后的正常化方法在反映转录基因激活时的准确性.

结论:

  • 开发的正常化策略有效地捕捉了克拉米迪亚在感染早期阶段转录组激活的真实程度.
  • 这些灵活和上下文意识的规范化原则可以应用于其他表现出不平衡基因表达动态的生物系统,例如细菌子发芽.