染色体重塑蛋白BPTF调节平面干细胞中的转录稳定性
bioRxiv : the preprint server for biology
|June 3, 2024
概括
基因组 H3 lysine 4 (H3K4me3) 的三甲基化招募了染色质重塑剂,影响了基因表达. BPTF蛋白对平面干细胞功能和转录至关重要,将H3K4me3与干细胞生物学联系起来.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 基因组H3 lysine 4三甲基化 (H3K4me3) 与基因表达有关,但其因果作用仍在争论中.
- 通过其子单元BPTF,染色体重塑复合体NuRF被H3K4me3招募来调节基因组可访问性.
- 之前关于BPTF招募机制的研究主要是在体外进行的.
研究的目的:
- 调查BPTF在平面干细胞中调节染色质可访问性和转录中的体内作用.
- 阐明BPTF在H3K4me3.3的背景下影响基因表达的机制.
主要方法:
- 直接从活的平面动物中分离干细胞.
- 在平面干细胞中BPTF功能的体内研究.
- 分析H3K4me3峰值特征 (例如宽度) 和它们与BPTF活动的关系.
主要成果:
- BPTF在基因促进器中起作用,并促进在Set1依赖的H3K4me3标记基因中的转录.
- 取决于集合的H3K4me3峰值比与MLL1/2.2.相关的峰值更宽.
- BPTF对平面干细胞生物学至关重要,其功能丧失模仿Set1敲击.
结论:
- BPTF和H3K4me3是体内转录的重要媒介.
- 在平面干细胞生物学中,BPTF发挥着至关重要的作用,其功能丧失的表型反映了Set1的敲击.
- 这些发现确立了H3K4me3,BPTF和干细胞功能之间的直接联系.
相关概念视频
Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Spreading of Chromatin Modifications
8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.3K
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K


