相关实验视频

Updated: Jun 21, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
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Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

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通过离子对逆相染色学量化ssDNA支架生产

A Rita Silva-Santos1,2, Sara Sousa Rosa1,2, Marco P C Marques3

  • 1iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal.

ACS omega
|June 3, 2024
PubMed
概括

这项研究引入了一种新的HPLC方法,用于精确量化DNA原始化中使用的DNA支架. 该方法改善了用于纳米技术应用的单链DNA (ssDNA) 的生产.

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相关概念视频

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...