一种简单,灵敏,更绿色的HPLC-DAD方法,用于同时分析两个新型素受体对手
Muzaffar Iqbal1, Nazrul Haq2, Mohd Abul Kalam2
1Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
ACS omega
|June 3, 2024
概括
一种新的,更绿色的高性能液态染色体二极管阵列探测器 (HPLC-DAD) 方法可以同时分析素受体对抗剂 (ORA),lemborexant (LMB) 和suvorexant (SUV). 这种经过验证的方法为这些失眠治疗提供了准确,灵敏和强大的量化.
科学领域:
- 分析化学 分析化学
- 药品分析 药品分析
- 绿色化学 绿色化学
背景情况:
- 素受体对抗剂 (ORAs) 是治疗失眠的新方法.
- 现有的分析方法缺乏同时量化和绿色化学原则,用于像lemorexant (LMB) 和suvorexant (SUV) 这样的ORAs.
研究的目的:
- 开发和验证一种简单,灵敏,更环保的高性能液态染色体二极管阵列探测器 (HPLC-DAD) 方法,用于同时对LMB和SUV进行定量分析.
- 为了评估开发的分析方法的绿色概况.
主要方法:
- 同时染色分离LMB和SUV使用C18列.
- 移动阶段:乙醇:10毫米KH2PO4缓冲器 (60:40v/v). 移动阶段:乙醇:10毫米KH2PO4缓冲器 (60:40v/v).
- 通过253nm的DAD进行检测;使用分析生态尺度 (AES),ChlorTox和AGREE评估绿色.
主要成果:
- 在LMB (125-5000 ng/mL) 和SUV (250-10,000 ng/mL) 实现了优异的线性.
- 该方法证明了准确性,精度,稳定性和高灵敏度.
- 绿色评价得到了很高的分数:AES (91),ChlorTox (1.14g) 和同意 (0.79).
结论:
- 成功开发了一种经过验证,更绿色的HPLC-DAD方法,用于同时量化LMB和SUV.
- 该方法适用于分析散装和合成混合物的LMB和SUV.
- 这种方法为ORA评估提供了一种一致且环保的分析解决方案.
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