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通过Bacillus thuringiensis-Mediated Defense Primining控制番茄虫虫病

Zahra Kiani Dehkian¹, Hengameh Taheri¹, Babak Pakdaman Sardrood²

¹Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

Iranian journal of biotechnology

|June 3, 2024

概括

细菌 thuringiensis (Bt) 起始增强番茄植物防御路径对虫. 这种生物控制方法改善了植物生长,减少了疾病的严重程度,显示出农业应用的前景.

科学领域:

植物病理学植物病理学
微生物学微生物学
生物控制生物控制

背景情况:

虫,是由 *Fusarium oxysporum* f. sp. 引起的番茄 (Solanum lycopersicum) 是一个全球主要的威胁番茄 (Solanum lycopersicum) 的生产.
这种疾病在全球显著降低了作物产量,需要有效的疾病管理策略.

研究的目的:

评估 *Bacillus thuringiensis* (Bt) 隔离物IBRC-M11096 对番茄植物中虫的生物控制潜力.
为了研究Bt化对番茄植物荷尔蒙防御通路和生长参数的影响.

主要方法:

量化逆转录PCR (qRT-PCR) 用于分析斯酸 (JA) 和酸 (SA) 途径基因的表达.
增长指数和疾病进展曲线下的区域 (AUDPC) 被评估以评估生物控制的有效性.
在用*Fol*接种之前,植物接受了Bt分离物IBRC-M11096的治疗.

主要成果:

用Bt进行细菌原始化显著增加了JA (*COI1*, *Pin2*) 和SA (*NPR1*, *PR1*) 途径基因的转录.
与仅用*Fol*接种的植物相比,Bt原始化导致AUDPC和疾病症状的减少.
经过处理的植物显著改善了芽 (新鲜和干燥重量) 和根 (干燥重量) 的生长.

结论:

用*Bacillus thuringiensis* (Bt) 隔离物IBRC-M11096进行番茄植物的原始化,可以增强植物的防御机制和生长.
Bt原料有效地降低了Fusarium的严重程度,并显示出作为西红生物控制剂的潜力.
需要进一步的实地研究来确认这种生物控制策略在各种环境条件下的有效性.

关键词:

在AUDPC中,AUDPC是AUDPC.基因表达基因表达基因表达荷尔蒙调节的防御系统启动启动启动电阻电阻电阻电阻电阻电阻

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