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了解通过IS26介导的抗微生物耐药性基因的快速传播

Na Tang^1,2, Dawei Wei^1,2, Yuan Zeng^1,2

¹State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China.

|June 3, 2024

概括

插入序列 (IS),就像IS26一样,对细菌中抗微生物耐药性基因 (ARG) 的传播有显著的贡献. 这些移动遗传元素显示出与抗生素使用相关的基因转移和扩张的强大潜力.

科学领域:

微生物学微生物学
遗传学遗传学是一种遗传学.
分子生物学分子生物学

背景情况:

插入序列 (ISs) 是已知的移动遗传元件,可促进细菌中抗微生物耐药性基因 (ARG) 的传播.
在ARG传输中,IS的特定角色和动态,特别是IS26,仍然不完全理解.

研究的目的:

调查IS26和ARG之间的关系.
分析携带ARG的IS26边界单位的传输特征和传输趋势.
了解IS26对抗生素耐药性传播的贡献.

主要方法:

在NCBIRefSeq数据库中,对2656个具有ARG的IS26边界单元从完整的细菌基因组进行生物信息分析.
这些单位内的ARG的识别和分类.
实验证实IS26在形成用于基因传播的复合转子子中的作用.

主要成果:

在12个抗生素类别中,IS26与124个ARG有关,占检测到的ARG的41.2%.
发现IS26结合的单元调解了Gammaproteobacteria中广泛的ARG传播,在细菌种群中具有很高的转移潜力.
IS26受限单位显示时间扩张与抗生素使用相关,可能是由于IS26的非特异性目标部位偏好.

结论:

IS26是细菌中ARG传播的重要驱动因素.
IS26受限单位表现出广泛的宿主范围和转移潜力,有助于抗生素耐药性的演变.
实验证据证实IS26能够通过复合转子子体形成来调解抗性基因的传播.

关键词:

在IS2626中使用.消费抗生素消费抗生素消费抗生素耐药性基因是抗生素耐药性的基因.横向基因转移是指水平基因转移.插入序列的插入序列.

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