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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Next-generation Sequencing03:00

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
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Updated: Jun 24, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

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为什么以及如何使用SeqCode

William B Whitman1, Maria Chuvochina2, Brian P Hedlund3

  • 1Department of Microbiology University of Georgia Athens Georgia USA.

mLife
|June 3, 2024
PubMed
概括
此摘要是机器生成的。

新的SeqCode允许使用基因组序列命名 prokaryotes,包括未培养的物种. 这一系统确保了每个 prokaryotic 物种的单一统一名称,无论是培养的还是非培养的.

关键词:
这是一个SeqCode.基因组序列的测序甲基因组-组装基因组的基因组.编号表中的名称.

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科学领域:

  • 微生物学 微生物学
  • 生物信息学是一种生物信息学.
  • 纳税学是一种分类学.

背景情况:

  • 国际核细胞命名法典 (ICNP) 传统上要求在培养群中进行类型菌株沉积.
  • 巨大的多样性 prokaryotes 仍然未培养,限制他们的正式分类学描述.
  • 基因组测序越来越多地用于 prokaryotic 的识别和分类.

研究的目的:

  • 介绍SeqCode (由序列数据描述的 Prokaryotes 的命名法) 作为一种新的 prokaryotic 命名系统.
  • 能够命名 prokaryotes,特别是未培养的物种,使用基因组序列作为类型材料.
  • 建立一个统一的分类学框架,用于培养和非培养的原始细胞.

主要方法:

  • 基因组序列在SeqCode下作为 prokaryotic 物种的命名类型.
  • 该SeqCode运行与ICNP类似的规则,但忽略了类型变异沉积的要求.
  • 一个在线注册表 (seqco.de) 便于从2022年1月1日开始有效发布名称.

主要成果:

  • SeqCode允许从环境DNA和那些难以培养的 prokaryotes 的永久命名.
  • 根据SeqCode有效发布的名称与ICNP名称在2022年后的优先级上进行竞争.
  • 每种 prokaryotic 物种都建立了一个单一的,独特的名称,整合了培养和未培养的生物体.

结论:

  • 由于未培养生物的流行,SeqCode对于命名大多数 prokaryotic 物种至关重要.
  • 它促进了有效的沟通和统一 prokaryotic 分类学的发展.
  • SeqCode社区鼓励参与这个分类系统的持续发展.