鉴定从大动脉硬化进展的大动脉狭窄的诊断基因
Chenxi Yu1, Yifeng Zhang1, Hui Chen2
1Department of Cardiovascular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People's Republic of China.
Journal of inflammation research
|June 3, 2024
概括
干白素-7 (IL-7) 和血管细胞粘附分子-1 (VCAM-1) 被确定为大动脉硬化症 (AVS) 进展到大动脉狭窄症 (AS) 的关键生物标志物. 这项研究为预防膜疾病进展提供了新的见解.
科学领域:
- 心血管生物学 心血管生物学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 大动脉硬化 (AVS) 可以发展为大动脉狭窄 (AS),这是一个严重的膜疾病,没有有效的医学疗法.
- 了解导致AVS-AS进展的分子机制对于制定预防策略至关重要.
研究的目的:
- 为了确定潜在的生物标志物用于AVS向AS的进展.
- 探索AVS和AS之间的基因表达差异,以发现新的治疗点.
主要方法:
- 从AVS和AS样本中分析基因表达数据集 (微阵列和RNA测序).
- 对差异表达基因 (DEGs) 的选,蛋白质-蛋白质相互作用 (PPI) 网络的构建,以及机器学习模型 (LASSO回归).
- 使用ROC曲线对诊断值的评估和免疫细胞透的分析;通过免疫组织化学验证蛋白质水平.
主要成果:
- 确定了330个DEG;IL-7和VCAM-1成为重要的候选生物标志物.
- 一个结合IL-7和VCAM-1的诊断模型显示诊断值高于0.75.
- 与AVS组织相比,AS组织显示M2巨细胞减少和gamma-delta T细胞增加,与验证的IL-7和VCAM-1蛋白水平较高.
结论:
- 鉴定出IL-7和VCAM-1是指向AVS-AS疾病进展的新生物标志物.
- 这项研究提供了第一个全面的基因表达分析,比较AVS和AS,可能指导未来疾病预防和管理的研究.
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