破解菌根菌的抗原代码:CFP-10/ESAT-6结核病皮肤测试和误导性的结果
Igor Krasilnikov1, Tatiana Lehnherr-Ilyina2, Milana Djonovic3
1"Biotechnology Developments" JSC, Moscow, Russia.
概括
使用CFP-10和ESAT-6的结核病 (TB) 抗原特异性测试可能会产生不准确的结果. 它们在真菌细菌中的存在并不能可靠地表明病原性,这表明标准的结核素皮肤检测可能更有效地诊断结核病.
科学领域:
- 菌株学 菌株学 菌株学
- 生物信息学是一种生物信息学.
- 免疫诊断 免疫诊断 免疫诊断 免疫诊断
背景情况:
- 结核病诊断工具通常依赖于检测抗原特异性免疫反应.
- 抗原CFP-10和ESAT-6通常用于这种结核病 (TB) 测试.
- 了解这些抗原的基因组分布对于诊断准确性至关重要.
研究的目的:
- 评估基于CFP-10和ESAT-6抗原的结核病测试的诊断潜力.
- 用生物信息学工具评估交叉反应性免疫反应.
- 调查CFP-10/ESAT-6序列与真菌菌菌病原性之间的关联.
主要方法:
- 来自NCBI基因组数据库的真菌细菌基因组组合的生物信息分析.
- 使用独立的BLAST和tblastn程序来检测CFP-10和ESAT-6序列.
- 在致病性和非致病性菌根菌株中分析了序列的存在.
主要成果:
- 在一些非致病性菌根细菌中发现了CFP-10和ESAT-6序列,而在一些致病性菌株中则没有.
- 在Mycobacterium结核复合体 (MTBC) 的物种和菌株中,序列存在不一致.
- 超过一半的非结核性菌根菌 (NTMB) 缺乏这些序列,符合它们的非致病性.
结论:
- CFP-10和ESAT-6序列的存在不是真菌菌菌病原性的可靠指标.
- 抗原特异性结核病测试可能会产生错误的阳性或错误的阴性结果,由于序列的变化.
- 标准的结核素皮肤测试,尽管其非特异性,可以提供更可靠的检测,与抗原特异性测试相比,致病菌菌.
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