全血多重测量使用基于电化学阿普他默的生物传感器
Élodie V d'Astous1, Philippe Dauphin-Ducharme1
1Université de Sherbrooke, Département de Chimie, 2500 boul. de l'Université, Sherbrooke, J1K 2R1, Canada. philippe.dauphin.ducharme@usherbrooke.ca.
Chemical communications (Cambridge, England)
|June 3, 2024
概括
这项研究引入了一种新的基于aptamer的新型电化学生物传感器,该生物传感器使用了oxazine ATTO 700. 这一创新使得真正的多重,连续和实时测量两个分子在未稀释的全血在单个电极上.
科学领域:
- 电化学 电化学 电化学
- 生物传感器技术技术
- 分子诊断学 分子诊断学
背景情况:
- 使用电化学生物传感器进行多重分子测量通常需要多个电极,这对新生儿和血友病患者等特定患者群体构成挑战.
- 现有的方法不适合在复杂的生物样本 (如全血) 中进行连续的实时分析.
研究的目的:
- 开发一种基于单个电极的电化学aptamer生物传感器,能够进行真正的多重检测.
- 为了能够在未稀释的全血中同时对两个不同的分子进行连续和实时的测量.
主要方法:
- 将氧染料ATTO 700纳入基于电化学的aptamer的传感器设计中.
- 测试传感器在未稀释的全血样本中同时检测两个目标分子的性能.
主要成果:
- 证明了使用单个电极实现"真实"多重检测的可行性.
- 成功地在未稀释的全血中对两个不同的分子进行了连续和实时测量.
结论:
- 开发的基于素ATTO 700的电化学吸收体生物传感器为多重分析提供了一种简化方法.
- 这种单电极系统为具有挑战性的生物矩阵实时诊断提供了一个有前途的平台,克服了多电极系统的局限性.
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