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Updated: Jun 24, 2025

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
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Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

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量子力学定量核磁共振使所有磁场的数字参考标准成为可能,并提高了qNMR的可持续性

Yuzo Nishizaki1, Naoki Sugimoto1, Toru Miura2

  • 1National Institute of Health Sciences, Kawasaki, Kanagawa 210-9501, Japan.

Analytical chemistry
|June 3, 2024
PubMed
概括

量子力学驱动的代功能化旋转分析 (HifSA) 创建了定量NMR (qHNMR) 的数字标准. 该方法通过各种磁场准确量化物质,包括立体异构体.

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Nuclear Magnetic Resonance (NMR): Overview

Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
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In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
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Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research.
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