Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

747
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
747
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.1K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.1K
Mass Spectrum01:23

Mass Spectrum

1.9K
A mass spectrum is the graphical representation of the relative abundance of the charged fragments in an analyte plotted against their mass-to-charge ratio (m/z). The plot's x axis represents the ratio of the mass of the charged fragment to the elementary charge it carries. The y axis of the plot represents the relative abundance of each charged species. The relative abundance is calculated from the signal intensity of each charged species recorded at the detector. The most intense signal...
1.9K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

301
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
301

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Agentic AI for Structural Elucidation and Discovery of Drug Metabolites from Mass Spectrometry Data.

bioRxiv : the preprint server for biology·2026
Same author

MALDI Tandem Mass Spectrometry for Colony-Based Dereplication of Natural Products.

bioRxiv : the preprint server for biology·2026
Same author

The Distribution of Xenobiotics of Terrigenous Origin in the Nearshore Waters of a Caribbean Island.

Environmental science & technology·2026
Same author

Early seed priming with closely related <i>Bacillus</i> strains induces divergent physiological and defense responses in melon.

Horticulture research·2026
Same author

Predicting Discrete Structural Transformations in Small Molecules from Tandem Mass Spectrometry.

bioRxiv : the preprint server for biology·2026
Same author

Conversion of CO<sub>2</sub> into valuable products: engineering the PirC-PGAM switch in cyanobacteria to direct carbon flux into desired products.

Microbial cell factories·2026

相关实验视频

Updated: Jun 24, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K

修改寻找器:双重质谱对齐使结构修改网站定位成为可能

Mohammad Reza Zare Shahneh1, Michael Strobel1, Giovanni Andrea Vitale2

  • 1Department of Computer Science and Engineering, University of California Riverside, 900 University Ave., Riverside, California 92521, United States.

Journal of the American Society for Mass Spectrometry
|June 3, 2024
PubMed
概括

ModiFinder使用双重质谱 (MS/MS) 数据识别小分子中的结构变化. 这种计算工具精确地定位了修改,加速了新化合物的发现.

更多相关视频

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

19.7K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

相关实验视频

Last Updated: Jun 24, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

19.7K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

科学领域:

  • 分析化学 分析化学
  • 计算化学的计算化学
  • 代谢学 代谢学 代谢学

背景情况:

  • 非定位的双重质谱法 (MS/MS) 是用于小分子分析的高通量方法.
  • 诸如MS/MS库搜索,模拟库搜索和分子网络等计算方法有助于化合物识别.
  • 在MS/MS数据中缺少网站特定结构修改信息的自动化方法.

研究的目的:

  • 介绍ModiFinder,一种用于定位小分子结构修改的新型计算工具.
  • 为了利用MS / MS在已知和未知化合物之间的光谱峰值对齐来识别修饰位.
  • 增强MS/MS模拟搜索和分子网络的能力,以发现新型化合物.

主要方法:

  • ModiFinder对齐了结构相关的已知和未知的小分子的MS/MS光谱.
  • 它侧重于移动的MS/MS碎片峰值,以识别含有修改的子结构.
  • 该工具合成了峰值对齐信息,以得分已知的分子上修改位点的可能性.

主要成果:

  • ModiFinder有效地定位了小分子中的结构修改.
  • 该方法通过精确确定修改点来增强未知的化合物的识别.
  • 在加速发现新化学实体的过程中证明了它的实用性.

结论:

  • ModiFinder提供了关于结构修改的特定地点信息,这是MS/MS数据分析中以前未得到满足的需求.
  • 这种工具显著提升了模拟搜索和分子网络的能力.
  • 莫迪Finder准备通过改进的结构阐释加速发现新型化合物.