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通过使用弱值放大功能的三明治分析实现高性能生物传感器.

Bei Wang1, Lizhong Zhang1, Gengyu Liang1

  • 1Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.

Talanta
|June 3, 2024
PubMed
概括

本研究介绍了一种新的无标签光学生物传感器,使用弱值放大用于敏感检测α-fetoprotein (AFP). 增强型传感器为早期疾病诊断和生物标志物监测提供了一个有前途的工具.

关键词:
3D打印的道 3D打印的道结合序列的结合序列.没有标签的传感器."三明治免疫试验" (英语:Sandwich immunoassay) 是一种免疫试验方法.弱值放大放大器的使用方法

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科学领域:

  • 生物医学工程 生物医学工程
  • 分析化学 分析化学
  • 光电学是指光电子产品.

背景情况:

  • 敏感地检测像α-fetoprotein (AFP) 这样的生物标志物对于早期疾病诊断至关重要.
  • 现有的生物传感方法往往在灵敏度,选择性方面面临限制,或需要复杂的标签程序.
  • 无标签的光学检测在简化测试程序和实现实时监控方面具有优势.

研究的目的:

  • 开发一种无标签的光学三明治免疫测试传感器,其灵敏度和可靠性提高.
  • 为了利用弱值放大 (WVA) 和总内部反射 (TIR) 来实现高性能生物传感.
  • 为了证明传感器在复杂的生物样本中检测低度生物标志物的能力.

主要方法:

  • 制造一个无标签的光学三明治免疫测试传感器,采用WVA和TIR原则.
  • 优化三明治测试结构,包括抗体结合序列,以提高响应性.
  • 使用3D打印道和化溶液进行可重复和稳定的测量.
  • 直接和三明治测定格式对α-fetoprotein (AFP) 检测的比较分析.

主要成果:

  • 在PBS溶液中实现了AFP的低检测极限 (LOD) 6.29 ng/mL (pM水平).
  • 与直接试验相比,三明治试验的高灵敏度和增强的响应性得到证明.
  • 在人血清中成功检测到AFP,证明了复杂样品的可行性和芯片的可重复使用性.
  • 对未配对抗原和同时检测多个生物标记物的选择性很好 (AFP,CEA,CA15-3).

结论:

  • 开发的基于WVA的无标签光学三明治免疫测试是一种高度敏感和可靠的生物传感平台.
  • 这种方法显示出早期疾病诊断和预防应用的巨大潜力.
  • 该传感器的简单性,可重复使用性和检测多个生物标记物的能力表明,与现有方法相比,在实际应用中更容易推广.