在Litopenaeus vannamei中,一种Peritrophin-44基因参与了对疾病的抗性
Li-Shi Liu1, Jin-Quan Fan1, Meng-En Zhang1
1Institute of Modern Aquaculture Science and Engineering (IMASE) / Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China.
International journal of biological macromolecules
|June 3, 2024
概括
围膜 (PM) 蛋白质LvPT44对于的生存至关重要. 它增强了对ER压力和白斑综合征病毒的抗病能力,强调了它对无脊椎动物健康的重要性.
科学领域:
- 海洋生物学 海洋生物学
- 无脊椎动物动物学
- 生物化学 生物化学
背景情况:
- 周膜 (PM) 是无脊椎动物 (如甲类动物) 中关键的中肠结构,保护病原体并帮助消化.
- 了解PM蛋白的功能是改善无脊椎动物健康和水产养殖的关键.
研究的目的:
- 为了识别和描述 Litopenaeus vannamei.的周围膜的蛋白质.
- 研究类生理学和疾病耐药性中的Peritrophin-44同源蛋白 (LvPT44) 的作用.
主要方法:
- 蛋白质组分析以确定Litopenaeus vannamei中的PM蛋白质.
- 基结合试验和免疫电子显微镜用于定位LvPT44.4.
- 在压力和病毒感染下分析LvPT44促进体活性和基因表达.
- RNA干扰 (RNAi) 来评估LvPT44在死亡中的功能作用.
主要成果:
- 确定了44种PM蛋白,包括LvPT44,它与素结合并局部化到PM.
- LvPT44的表达受到STAT和c-Jun的调节,并由ER压力和白斑综合征病毒 (WSSV) 诱导.
- 在ER压力或WSSV感染后,LvPT44 Knockdown显著增加了的死亡率.
结论:
- LvPT44是围膜的功能组成部分,在疾病耐药性方面发挥着重要作用.
- 在ER压力和WSSV感染期间,LvPT44对于的生存至关重要,这表明它对天生的免疫的重要性.
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