来自Anoxybacillus karvacharensis K1的耐洗剂α-氨酶及其基于乳清奶粉的生产
Diana Ghevondyan1,2, Tigran Soghomonyan3, Pargev Hovhannisyan1,4,5
1Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Alex Manoogian 1, 0025, Yerevan, Armenia.
Scientific reports
|June 3, 2024
概括
这项研究使用乳清作为乳制品副产品,从Anoxybacillus karvacharensis中产生复合性α-氨酶. 该酶显示出高活性和稳定性,使其适合生物技术应用.
科学领域:
- 生物技术和生物化学
- 酵素工程是什么? 酶工程是什么
- 工业微生物学 工业微生物学
背景情况:
- 农业工业废物利用对可持续生物技术至关重要.
- 乳清是乳制品加工的副产品,是微生物发酵的潜在低成本媒介.
- 重组酶为工业应用提供了量身定制的特性.
研究的目的:
- 为了产生和表征从Anoxybacillus karvacharensis菌株K1.1.中复合的α-氨酶.
- 为了利用乳清作为一种成本效益高的生长介质,用于酶生产.
- 评估酶的生物化学特性和工业用途的稳定性.
主要方法:
- 在使用乳清介质的Anoxybacillus karvacharensis中进行重组阿尔法-氨酶的生产.
- 酶净化和表征,包括特定活性,最佳温度和pH值.
- 在各种条件下 (温度,pH值,盐,添加剂) 进行稳定性测试.
- 使用可溶性粉确定动力参数 (Km,Vmax).
主要成果:
- 纯化西丁标记的α-氨酶显示出高特异活性 (1069.2 U mg-1).
- 在55°C和pH值6.5的最佳活性,具有良好的热稳定性 (在55°C3小时后70%的活性).
- 对小麦粉的高活性和特殊的盐分耐受性 (高达2.5M).
- 常见的洗剂,金属离子或还原剂没有显著的抑制;CaCl2的轻微激活.
- 有利的生产条件包括24小时用β-葡萄糖酶对酸乳糖进行处理.
结论:
- 阿诺西巴塞勒斯karvacharensis可以有效地使用乳清产生强大的重组阿尔法-氨酶.
- 该酶的稳定性和对各种条件的耐受性使其成为工业应用的有希望的候选者.
- 通过重组酶生产来增值乳清是一种可持续的生物技术方法.
关键词:
亚诺西菌 karvacharensis karvacharensis 亚诺西菌 karvacharensis 亚诺西菌酸性乳酪糖是什么意思 酸性乳酪糖是什么意思生物化学表征 生物化学表征克隆 克隆就是克隆.表达方式表达方式表达α-氨基酸氨基酶的使用.更多相关视频
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