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相关概念视频

Synthetic Biology02:55

Synthetic Biology

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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
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Export of Mitochondrial and Chloroplast Genes02:19

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A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Genetic Material01:20

Genetic Material

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Within the human body, a complex and detailed system of trillions of cells works in unison to sustain life. Each cell houses a nucleus, which contains 46 chromosomes divided into 23 pairs. Chromosomes are highly coiled structures made of the genetic material DNA. These chromosomes are essential carriers of genetic information, with half inherited from the mother through her egg and the other half from the father's sperm, combining to create the unique genetic makeup of an individual.
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使用GenoDesigner设计一个合成基因组.

Wenfei Yu1,2, Shuo Zhang2,3, Shijun Zhao1,2

  • 1Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

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合成多细胞植物基因组是复杂的. 我们介绍了设计原则和GenoDesigner平台,以加速Physcomitrium patens基因组的新合成.

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科学领域:

  • 合成生物学 合成生物学
  • 植物基因组学 植物基因组学
  • 基因组工程是基因组工程.

背景情况:

  • 在微生物中,de novo基因组合成已取得显著的进步,但由于基因组复杂性和技术挑战,在多细胞植物中滞后.
  • 植物基因组在尺寸,结构,基因调节和表达方面存在独特的障碍,阻碍了合成努力.

研究的目的:

  • 为新生合成Physcomitrium patens (土) 基因组建立自下而上的设计原则.
  • 开发一个公开可访问的在线平台,GenoDesigner,以帮助基因组序列操纵和促进国际合作.

主要方法:

  • 概述基因组合成的自下而上的设计原则.
  • 开发和推出GenoDesigner在线平台,具有用于操纵大型基因组序列的图形界面.

主要成果:

  • 基因设计者平台能够高效地操纵基因组序列,直到千兆基基层.
  • 开发的原则和平台为加速P. patens基因组合成提供了路线图.

结论:

  • 基因设计者平台和设计原则对于推进Physcomitrium patens基因组的新合成至关重要.
  • 这项工作为未来的植物基因组合成项目提供了基础参考.