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在小鼠中,生长激素受体的干扰减少了骨关节炎和冠状细胞缩

Huanhuan Liu^1,2,3, Trent Davis¹, Silvana Duran-Ortiz^1,4,5

¹Ohio University Heritage College of Osteopathic Medicine, Ohio University, Athens, Ohio, USA.

|June 3, 2024

概括

生殖系和诱导性生长激素受体 (GHR) 缺乏保护小鼠免受与衰老相关的关节炎. 完全的GHR缺陷提供了比部分的成年期缺陷更强大的关节保护.

科学领域:

内分泌学和新陈代谢学
肌肉骨生物学肌肉骨生物学
衰老研究研究衰老研究

背景情况:

过度的生长激素 (GH) 与关节退化有关.
破坏GH信号传递,特别是GH受体 (GHR) 缺乏在关节健康中的作用仍然在很大程度上是未知的.
了解GHR的影响对于开发骨关节炎 (OA) 疗法至关重要.

研究的目的:

为了研究生殖系 (GHR^-/-) 和成人诱导 (iGHR^-/-) GHR缺乏对小鼠关节病理学的影响.
评估 GHR 干扰对与年龄相关的骨关节炎发展和进展的影响.

主要方法:

从GHR^-/-和野生型 (WT) 小鼠的膝关节组织学分析.
微计算机断层扫描 (μCT) 和膝关节关节组织学评估来自iGHR^-/-和WT小鼠.
冠状细胞缩 (COLX) 和软骨退行 (ADAMTS-5) 标志物的免疫组织化学.

主要成果:

GHR^-/-小鼠表现出光滑的关节表面和没有退化的迹象,与WT相比,OARSI和曼金得分明显较低.
iGHR^-/-小鼠对与衰老相关的OA表现出适度的保护,曼金得分明显较低,但OARSI得分与WT相比没有显著差异.
两种GHR-/-和iGHR-/-小鼠都有较少的超性红细胞 (COLX+),但没有观察到ADAMTS-5染色的差异.

结论:

无论是生殖系还是成年人诱导的GHR缺乏,都会对老化相关的老鼠关节炎进行保护.
完全的GHR缺乏 (GHR^-/-) 提供比成人诱导的GHR缺乏 (iGHR^-/-) 更有效的保护.
GHR信号传递在与年龄相关的关节退化病变发生过程中发挥着重要作用.

关键词:

冠状细胞是什么意思冠状细胞是什么意思生长激素受体生长激素受体过度缩过度缩过度缩骨关节炎是一种骨关节炎.

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