强烈的消毒程序对从猪肉加工厂的地板下水道中回收的细菌群体的影响
Joseph M Bosilevac1, Manita Guragain1, Darryll A Barkhouse2
1U.S. Department of Agriculture, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE, United States.
Frontiers in microbiology
|June 4, 2024
概括
猪肉植物的强化消毒 (IS) 暂时减少了环境细菌,但微生物群落往往会迅速复苏,有时会改变组成. 这些治疗方法可能无法有效地消除病原体或长期破坏生物.
科学领域:
- 食品安全 食品安全
- 微生物学 微生物学
- 环境科学 环境科学
背景情况:
- 猪肉加工厂每6-12个月使用一次强烈的消毒 (IS).
- IS的目标是破坏庇护病原体和腐烂生物体的生物膜.
- 在IS后,环境微生物的短期影响和恢复率尚不清楚.
研究的目的:
- 调查IS对美国猪肉加工厂环境微生物的影响.
- 为了确定IS后细菌社区恢复的速度.
- 评估IS在减少特定微生物数量及其耐药性质方面的有效性.
主要方法:
- 从地板下水道,冷却器和切割桌附近收集的环境拭子样本.
- 微生物分析包括总美索菲尔人数 (TMC),总心理人数 (TPC) 和指标细菌.
- 评估了生物膜形成能力,四级化合物 (QAC) 耐受性和对*Salmonella enterica*的保护.
- 细菌群体的组成被分析使用枪枪元基因组测序.
主要成果:
- IS降低了TMC和TPC,某些地区的水平在2-3天内恢复到IS前的水平.
- 冷却器中的微生物减少变化很大 (2-4 Log10),需要 2-4 周的恢复时间.
- 制造线的结果是混合的;一个工厂在IS后显示出显著的减少 (4-6Log10).
- 生物膜对QAC和*沙门氏菌*保护的耐药性因植物和位置而异.
- IS减少了微生物多样性,导致社区组成发生了变化,有时会长期存在.
结论:
- 猪肉加工厂的强化消毒 (IS) 对环境微生物具有可变且往往短暂的影响.
- 细菌群体可以快速恢复,并且IS可能导致微生物组成的持续变化.
- 在破坏病原体和腐烂生物体方面,IS的有效性可能是有限的,质疑其整体影响.
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