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Teng Long¹, Pratyajit Mohapatra¹, Sydney Ballou¹

¹Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT, United States.

Frontiers in cellular neuroscience

|June 4, 2024

概括

昆虫的气味共受体维持调受体mRNA水平,但似乎不必对Drosophila的嗅觉神经元生存至关重要. 大多数嗅觉神经元即使在没有共同受体依赖调受体的情况下也存在.

科学领域:

神经科学是一个神经科学.
昆虫的嗅觉是昆虫的嗅觉
分子生物学分子生物学

背景情况:

昆虫利用气味受体 (OR) 和离子受体 (IR) 来检测气味.
特定的共同受体子单元 (Orco,Ir8a,Ir25a,Ir76b) 对于OR和IR功能至关重要.
以前的研究表明,共同受体可能对某些昆虫的嗅觉神经元存活至关重要.

研究的目的:

为了研究共受体在保持Drosophila的嗅觉神经元存活中的作用.
评估共受体突变对调受体基因表达的影响.
识别受OR和IR共受体缺失影响的其他天线基因.

主要方法:

利用天线转录组来测量调受体表达,作为嗅觉神经元类生存的代理.
基因表达在共同受体突变的多索菲拉 (Orco,Ir8a,Ir25a,Ir76b) 与野生类型对照进行了比较.
进行了差异基因表达分析,以确定受影响的天线基因.

主要成果:

奥尔科共受体突变导致许多OR调受体转录的表达减少,与年龄相关的影响.
尽管调受体表达减少,但Orco突变体中的大多数嗅觉神经元幸存下来,显示最小的亡.
Ir8a和Ir25a突变降低了IR调受体表达,但相关的神经元在很大程度上持续存在;Ir76b没有显著的影响.

结论:

气味共受体对于维持许多相关调受体的mRNA水平至关重要.
大多数Drosophila嗅觉神经元独立于共受体介导的调受体表达而存活.
对于神经元生存的共同受体的必要性可能因昆虫物种而异.

关键词:

这种植物是Drosophila.一个天线天线天线.嗅觉嗅觉是一种嗅觉.嗅觉感官神经元的神经元接收器接收器接收器接收器转录组 (transcriptome) 是一个转录组.

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