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对五种表型方法进行比较评估,用于识别产生卡巴酶的细菌群Enterobacteriaceae:一种修改的卡巴酶检测试验

Hamid Solgi^1,2, Ali Badamchi³, Fereshteh Shahcheraghi⁴

¹Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Microbiology spectrum

|June 4, 2024

概括

快速检测产生卡巴酶的肠道细菌 (CPE) 是至关重要的. 修改后的Carba NP测试显示出高灵敏度 (95.06%) 和特异性 (94.44%),使其成为确定这些耐药细菌的可靠表型方法.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
抗微生物耐药性抗微生物耐药性

背景情况:

碳酶生成的肠杆菌 (CPE) 在医疗保健环境中构成重大威胁.
快速检测CPE对于感染控制和患者管理至关重要.
现有的检测方法在灵敏度和特异性上有所不同.

研究的目的:

评估五种表型方法的性能,以检测Enterobacteriaceae中的碳酶生产.
为了确定最敏感和最具特异性的CPE检测快速测试.
为了比较内部Carba NP (iCarba NP),修改-Carba NP,E-Test MBL,修改的Hodge测试 (MHT) 和商业磁盘测试的准确性.

主要方法:

检测了99个临床和直肠Enterobacteriaceae分离物 (81个具有已知的碳烯酶基因,18个没有).
进行了五项表型测试:iCarba NP,修改-Carba NP,E-Test MBL,MHT和商业磁盘测试.
对iCarba NP和修改Carba NP的结果在多个时间点 (1分钟至2小时) 读取.

主要成果:

修改后的Carba NP测试显示了最高的灵敏度 (95.06%) 和良好的特异性 (94.44%).
iCarba NP的敏感度为78.87%,具有100%的特异性.
电子测试MBL (91.30%/100%) 和氨基烯酸协同作用测试 (100%/97.94%) 也显示出高性能.

结论:

修改的Carba NP测试是一种可靠和敏感的表型方法,用于预测产生卡巴酶的细菌.
准确和快速的实验室检测CPE对于监测和临床决策至关重要.
修改后的Carba NP测试被推作为CPE检测的选方法.

关键词:

它们是Enterobacteriaceae的细菌.碳烯质量的减少现型检测检测现型检测

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