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卡卢斯特异性的CRISPR/Cas9系统增加了玉米中的遗传基因突变

Yuan Shi¹, Jing Wang¹, Tante Yu¹

¹Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China.

|June 4, 2024

概括

在玉米中,一种新的特定于的CRISPR/Cas9系统减少了不必要的体质突变,改善了作物育种所需遗传变化的继承. 这个系统增强了跨代突变的稳定性.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农作物科学农作物科学

背景情况:

克里斯普尔/Cas9是一种强大的作物遗传育种工具.
无处不在的CRISPR/Cas9表达导致众多体质突变,阻碍了遗传突变的检测.
针对特定组织的基因编辑可能会提高效率.

研究的目的:

在玉米中开发和评估一种特定于的CRISPR/Cas9 (CSC) 系统.
为了将CSC系统与无处不在的CRISPR/Cas9 (UC) 系统进行比较.
评估体突变对遗传突变检测和稳定性的影响.

主要方法:

使用玉米特异性促进体 (pZmCTA1,pZmPLTP) 构建了一个CSC系统,以驱动Cas9表达.
针对bZIP转录因子Opaque2 (O2) 基因进行编辑.
利用突变的高通量跟踪 (Hi-TOM) 进行编辑效率分析.
生成的转基因植物和分析T0和T1代的突变.

主要成果:

与UC系统相比,CSC系统在calli中产生了更多的目标基因突变.
CSC系统在T0幼苗中产生了较少的基因突变,但减少了体质突变的影响.
来自CSC系统的T1突变几乎100%是从T0植物遗传的,而UC系统的6.3-16.7%.

结论:

该CSC系统显著减少了体质突变,并增强了玉米中稳定,可遗传的突变的产生.
这个系统提供了一个有前途的方法,可以在各种作物中精确地进行基因育种.
改进的遗传突变检测有助于培养具有所需特征的作物.

关键词:

齐亚梅斯 (Zea mays) 是一种植物.这就是CRISPR/Cas9的作用.农作物遗传育种农作物遗传育种可以遗传的基因突变.

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