特里顿X-100降低Enterococcus faecalis的Ag+抗性的机制
Silei Lv1, Mengting Duan1, Bing Fan2
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.
特里顿X-100通过破坏细胞膜来增强银离子对Enterococcus faecalis的抗菌作用. 这种组合疗法证明对正常和抗银菌株都有效.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- Enterococcus faecalis对银离子 (Ag+),一种常见的抗菌剂,表现出耐药性.
- 作为一种非离子表面活性剂,Triton X-100 (TX-100) 正在研究其克服这种抗性的潜力.
研究的目的:
- 阐明TX-100降低E. faecalis. Ag+抗性的机制.
- 评估TX-100和Ag+对正常和诱导的Ag+耐药E. faecalis (AREf) 的联合抗菌疗效.
主要方法:
- 确定Ag+的最小抑制度 (MIC) 和最小杀菌度 (MBC) 与/或没有TX-100.
- 传输电子显微镜 (TEM) 用于形态分析.
- 感应合血质谱法 (ICP-MS) 检测细胞内Ag+度.
- 流细胞计测试以评估细胞膜的潜力和完整性.
主要成果:
- 0.04% TX-100最大化了Ag+增强的抗菌作用.
- TX-100通过改变E. faecalis膜结构,完整性,潜力和透性来促进Ag+的进入.
- 结合的TX-100 + Ag+证明了对敏感和诱导的AREf的增强疗效.
结论:
- TX-100破坏了E. faecalis的膜,增强了Ag+的透力,克服了Ag+的抵抗力.
- TX-100和Ag+的协同效应提供了对抗E. faecalis感染的有希望的策略,包括耐药菌株.
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