在适应性金属有机框架中的生物灵感异构协调用于DNA表观遗传修饰检测
Zhongyu Wei1,2, Long Yu1,3, Yumin Feng2
1Department of Thyroid and Breast Surgery, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
Analytical chemistry
|June 4, 2024
概括
研究人员开发了一种新的低成本金属有机框架 (MOF) 探测策略. 这种方法有效地检测DNA上的表观遗传修饰,为早期疾病诊断铺平了道路.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 金属有机框架 (MOF) 在模仿自然协调系统方面具有独特的优势.
- 引入第二个链接器,以指蛋白为灵感,可以增强MOF的功能.
研究的目的:
- 开发一种基于MOF的新型传感策略,用于检测表观遗传修饰.
- 创建一种具有成本效益和高度敏感的方法,用于早期疾病诊断.
主要方法:
- 对12种MOF物种和6种第二链接器进行系统选,以确定最佳的消散系统 (Co-BDC-NO2和o-phenylenediamine).
- 利用表观遗传修饰的碳活化和金属离子选,使得DNA链上可适应的Co-MOF生长.
- 开发一种双模消散信号生成,用于敏感检测5-基甲基和5-甲基.
主要成果:
- 确定了一个Co-MOF/oPD散热系统,迅速产生一个高光热复合体 (η = 36.9%).
- 该策略成功地将DNA表观遗传信息转化为放大金属离子信号.
- 实现了对5-基甲基细胞因子 (LOD = 0.02%) 和5-甲基细胞因子 (LOD = 0.025‰) 的高灵敏度检测.
结论:
- 建立了一种低成本 (<0.01美元/样本) 的方法来量化全球表观遗传修饰.
- 开发的基于MOF的传感范式为分子识别和信号传导提供了新的方法.
- 这项工作显著推进了基于表观遗传修饰的早期疾病诊断.
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