当表面增强的拉曼光谱与复杂的生物流体相遇时:用于可靠和全面的表征的新表示策略
Chang He1, Fugang Liu1, Jiayi Wang2
1State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Shanghai Jiao Tong University, 200030, Shanghai, PR China.
Analytica chimica acta
|June 4, 2024
概括
这项研究引入了一种新的表面增强拉曼光谱 (SERS) 光谱集方法,用于全面的生物流体分析. 这种方法捕获了完整的分子信息,在复杂的样本表征方面表现优于传统的单个或平均光谱.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 生物分子分析
背景情况:
- 表面增强拉曼光谱 (SERS) 为分子检测提供了高特异性和灵敏度.
- 鉴定复杂的生物流体,如细胞溶解物和血清,由于众多的生物分子和度变化,具有挑战性.
- 使用单个或平均光谱的传统SERS方法无法捕获复杂生物流体的全部分子信息.
研究的目的:
- 开发一种新的表征策略,使用SERS来表征复杂生物流体中的完整分子信息.
- 引入瓦瑟斯坦距离 (WD) 作为评估SERS光谱集的可靠方法,克服传统化学测量的局限性.
主要方法:
- 从无序的多个SERS光谱构建一个光谱集,以表示复杂的生物流体样本.
- 使用瓦瑟斯坦距离 (WD) 进行SERS光谱集的定量评估,独立于光谱排序.
- 使用细胞溶解物和人体血清进行实验验证,以验证可重复性,均性,可重复性和核心性效应.
主要成果:
- 该SERS光谱集成功捕获了详细的分子信息和空间/时间分布特征.
- 瓦斯斯坦距离在量化评估光谱集及其属性方面表现出优越性.
- 实验证实了SERS光谱集方法的可重复性,统一性和可重复性.
- SERS光谱集有效地区分了不同的人类血清类别,并提高了前列腺癌分类准确度.
结论:
- 拟议的SERS光谱集是获得生物样本综合信息的可靠表示.
- 这种方法在可重复性,统一性和可重复性方面比单个或平均光谱具有显著的优势.
- 瓦瑟斯坦距离的整合增强了复杂生物流体的表征,巩固了SERS在生物分析中的作用.
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