对血红素和血红素进行定量分析,以检测血管内血液溶解
Marie-T Hopp1, Sonali M Vaidya2, Karina M Grimmig2
1Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn, D-53121, Bonn, Germany; Department of Chemistry, Institute for Integrated Natural Sciences, University of Koblenz, D-56070, Koblenz, Germany.
Analytica chimica acta
|June 4, 2024
概括
在诊断血管内血液溶解时,不稳定的血红和血红蛋白量化是至关重要的. 目前的方法难以区分血红素形式,需要改进的技术来进行准确的临床评估.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 血液学 血液学 血液学
背景情况:
- 血管内血解释放出血红蛋白和不稳定血,导致血栓前和炎症前作用.
- 已知heme在不良影响中的作用,但未经临床监测;相反,Haptoglobin和血红蛋白被使用.
- 除了血红蛋白之外,定量可变的血红素对于完整的血液溶解状态图片至关重要.
研究的目的:
- 系统地分析和比较现有的临床应用的可变血量化技术.
- 评估不同方法区分血红蛋白结合的血红素和可变血红素的能力.
- 开发和介绍一种新的综合方法,用于同时确定血红蛋白和可变血红蛋白.
主要方法:
- 评估两种商业测定和五种常见方法 (光谱,HPLC,质谱) 用于血质量测定.
- 对血红蛋白量化技术 (Harboe,SLS,Hemastix®) 的选择性和干扰性进行分析.
- 开发和应用一种用于血样本的新型组合光谱方法.
主要成果:
- 直接和间接的方法都有效量化了血红素 (∼0.02-45μM) 和血红素 (∼0.002-17μM).
- 没有任何一种方法可以可靠地区分血红蛋白结合的血红素和不稳定的血红素.
- 一种新的综合方法成功应用于人类和猪血.
结论:
- 需要改进的技术来区分可变和与蛋白质结合的血,以便早期检测血管内血解.
- 结合两种光谱方法的新策略提供了一个可靠的,用户友好的工具.
- 这种方法有助于诊断血管内血解以及通过确定血血红蛋白和血红蛋白水平来进行基础生物医学研究.
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