在小鼠腹部海马体中的Histone H1x与,但不会引起对压力的行为适应
R Kijoon Kim1, Natalie L Truby1, Gabriella M Silva1
1Department of Anatomy and Neurobiology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.
Translational psychiatry
|June 4, 2024
概括
腹部海马H1x蛋白水平升高与小鼠的压力易感性相关. 然而,过度表达H1x并没有导致行为变化,这表明它与压力适应有关,但不是唯一的驱动因素.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 行为科学 行为科学
背景情况:
- 应激弹性和易感性涉及腹部海马体 (vHipp) 中的差异性蛋白质表达.
- 链接组合素H1x (H1x) 蛋白水平在容易受到压力的小鼠和耐压力的小鼠中显著不同.
- H1x在异色染色体紧缩中的作用表明了压力诱导的行为分歧的潜在机制.
研究的目的:
- 调查vHipp H1x蛋白水平升高是否直接导致慢性社会压力的行为适应.
- 测试假设增加的H1x阻碍了亲弹性转录变化和弹性的发展.
主要方法:
- 小鼠经历了慢性社会失败压力 (CSDS) 或单一住房,并被行为分类为易感或弹性.
- 用病毒载体在小鼠的vHipp中过度表达 (OE) H1x或控制GFP.
- 在病毒转导后,对小鼠进行了行为测试 (社交互动,焦虑,记忆).
主要成果:
- 易受压力影响的小鼠在经过压力后显示出vHipp H1x蛋白水平升高.
- 与对照组相比,vHipp中的病毒H1xOE并没有在易受感染,弹性或单独养的小鼠中诱导显著的行为适应.
- 在H1x OE和GFP组之间没有观察到社会行为,类似焦虑的行为或依赖记忆的任务的显著差异.
结论:
- 升高的vHipp H1x蛋白水平与对慢性社会压力的敏感性有关.
- H1x过度表达本身不足以推动对压力的行为适应的发展.
- 虽然H1x与压力易感性有关,但它似乎不是行为分歧的单一因果因素.
相关概念视频
02:10The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:30Histone Variants at the Centromere
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
01:19The Nucleosome
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
01:12The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...