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蛇的染色体层次基因组组合蒙古罗拉菲迪亚杜米利亚 (Raphidioptera: Raphidiidae)

Rongrong Shen^1,2,3, Terrence Sylvester^2,3, Na Ra Shin^2,3

¹Department of Entomology, China Agricultural University, Beijing, 100193, China.

Scientific data

|June 4, 2024

概括

我们向您介绍了蛇Mongoloraphidia duomilia的第一个染色体级基因组组. 这一资源有助于理解昆虫中完全变形的进化过程.

科学领域:

基因组学就是基因组学.
进化生物学进化生物学
昆虫学昆虫学是一门学科.

背景情况:

蛇 (Raphidioptera) 是一个整体代谢的昆虫类,对于研究完整变形的进化至关重要.
尽管它们具有进化意义,但蛇的物种多样性和基因组资源有限.

研究的目的:

为了生成一个高质量的,染色体水平的参考基因组组装亚洲特有的蛇Mongoloraphidia duomilia.
为Neuropterida和Holometabola.提供基因组资源进行比较分析.

主要方法:

利用PacBio的HiFi和Hi-C测序数据从女性标本.
采用先进的生物信息管道来进行基因组组装和注释.
将组件定到染色体水平.

主要成果:

实现了653.56 Mb的基因组组合,其中97.90%的基因被在13个染色体上.
脚手架N50为53.50Mb,而BUSCO的完整性达到了97.80%.
确定了599个非编码RNA,并预测了11,141个蛋白质编码基因.

结论:

蒙古罗拉菲迪亚杜米利亚基因组代表了昆虫基因组学的重大进步.
这种资源将促进对昆虫类Neuropterida和Coleopterida之间的进化过渡的研究.
它有助于理解完整变形的基因组基础.

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