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西诺因通过抑制MMP9和炎症性细胞因子表达来治疗类风湿性关节炎:生物信息学分析和实验验证验证

Jinfang Luo¹, Yi Zhu¹, Yang Yu¹

¹Department of Basic Medicine, Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, People's Republic of China.

Scientific reports

|June 4, 2024

概括

西诺因 (SIN) 通过减少矩阵金属蛋白酶9 (MMP9) 和炎症性细胞因子,可以治疗类风湿性关节炎 (RA). 这项研究确定了MMP9作为关键的RA生物标志物,并验证了SIN的抗炎作用.

科学领域:

免疫学和风湿病学
计算生物学和生物信息学
药理学药理学是指药理学的学科.

背景情况:

类风湿性关节炎 (RA) 是一种慢性自身免疫性疾病,导致关节炎症和损伤.
西诺 (Sinomenine,简称SIN) 是中国批准用于治疗RA的抗炎药物.
在RA中SIN的精确抗炎机制需要进一步阐明.

研究的目的:

通过生物信息学和实验方法,研究在类风湿性关节炎 (RA) 中sinomenine (SIN) 的抗炎作用.
确定关键的生物标志物和信号通路,涉及RA病原和SIN的治疗效果.
在一个体外炎症模型中验证SIN的疗效.

主要方法:

获取和分析六个基因表达综合 (GEO) 微阵列数据集,用于RA.
鉴定差异表达基因 (DEGs),免疫细胞透 (CIBERSORT) 和M1巨相关基因 (WGCNA).
对SIN向基因的生物信息预测,枢纽基因选择 (cytoHubba, LASSO),ROC曲线分析,分子对接和体外验证 (RAW264.7细胞,西部斑点,ELISA).

主要成果:

矩阵金属蛋白酶9 (MMP9) 被确定为RA中关键的M1巨相关生物标志物.
MMP9与IL-17和TNF信号通路有关.
在LPS诱导的RAW264.7细胞中,Sinomenine (SIN) 通过抑制MMP9过度表达和促炎细胞因子 (TNF-α,IL-6) 证明了治疗疗效.

结论:

这项研究强调MMP9作为潜在的关键基因和类风湿性关节炎的治疗点.
西诺因 (SIN) 通过调节MMP9和关键的炎症性细胞因子,表现出抗炎性质.
作为一种潜在的具有成本效益的抗炎药物,SIN在 RA 治疗中表现有前途.

关键词:

生物信息学是一种生物信息学.在IL-6的研究中,IL-6被认为是IL-6的基因.在M1巨细胞中.在MMP9中,MMP9是MMP9.类风湿性关节炎类风湿性关节炎类风湿性关节炎这就是"sinomenine".这就是TNF-α.

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