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脊尾白Exopalaemon carinicauda的染色体层次的基因组组合

Jiajia Wang^1,2, Jianjian Lv^1,2, Miao Shi³

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, 266071, China.

Scientific data

|June 4, 2024

概括

使用先进的测序创建了Exopalaemon carinicauda,一个关键的水产养殖的高质量基因组组. 该资源将促进类动物的分子育种和功能基因组学研究.

科学领域:

基因组学就是基因组学.
水产养殖是水产养殖的一种方式.
甲动物生物学甲动物生物学

背景情况:

(Exopalaemon carinicauda) 是一种欧热和欧类,对中国东部的多种养殖池至关重要.
由于其生态和经济意义,它被认为是类研究的潜在模型生物.

研究的目的:

为了生成一个高质量的,染色体水平的基因组组装Exopalaemon carinicauda.
为未来的分子育种和功能基因组学研究提供一个有价值的基因组学资源.

主要方法:

使用PacBio HiFi和Hi-C测序技术进行基因组组装.
使用生物信息学分析用于组装大小,contig/scaffold N50,基因预测和注释.
通过BUSCO分析评估基因组质量和异合性/重复序列比例.

主要成果:

实现了5.86Gb的染色体级基因组组合,使用138.24Mb的N50支架.
定了95.29%的序列到45个伪染色体,确定了92.89%的保存的正确基因.
预测了44,288个编码蛋白质的基因,其中70.53%具有功能性注释,并指出高异构性 (2.62%) 和重复含量 (71.49%).

结论:

成功组装了一个复杂的,染色体规模的基因组为Exopalaemon carinicauda.
该基因组代表了类研究的重大进步,促进了基因改进和功能研究.

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Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.