在PARP14和PARP9/DTX3L中,调节干扰素诱导的ADP-ribosylation
Pulak Kar1,2, Chatrin Chatrin1, Nina Đukić1
1Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, UK.
The EMBO journal
|June 4, 2024
概括
研究人员开发了研究单-ADP-ribosylation (ADPr) 的新方法,揭示了 PARP14 .
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 聚 ((ADP-ribose) 聚合酶 (PARP) 催化ADP-ribosylation (ADPr) 调节细胞过程.
- 由于检测方法有限,以前很难研究PARP依赖的单-ADP-ribosylation.
研究的目的:
- 开发和使用敏感的方法来检测单一ADP-ribosylation.
- 研究PARP酶在干扰素 (IFN) 诱导的信号传递中的作用.
- 探索IFN响应中的ADPR和无处不在的相互作用.
主要方法:
- 利用一种改进的抗体来敏感地检测单-ADP-ribosylation.
- 视觉化的内源性IFN诱导的ADP-ribosylation.
- 研究了PARP9,DTX3L和SARS-CoV-2的蛋白质-蛋白质相互作用和酶活性 Mac1.
主要成果:
- 确定了PARP14作为IFN诱导的ADP-ribosylation中的一个关键酶.
- 证明SARS-CoV-2 Mac1可以逆转PARP14介导的信号传输.
- 阐明了PARP9和DTX3L在调节PARP14活动中的调节作用.
- 在IFN响应中首次可视化了ADPR依赖的无处不在.
结论:
- 新的方法推动了IFN信号传输中ADP-ribosylation和ubiquitylation的研究.
- PARP14是IFN反应中的关键酶,被SARS-CoV-2Mac1.1.等病毒因素所准.
- 了解这些途径可以了解病原体逃避机制.
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