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对携带单个与堆叠的抵抗基因对俱乐部根基因进行的卡诺拉的比较转录组分析

Rui Wen¹, Tao Song¹, Bruce D Gossen¹

¹Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon SK, Canada.

Frontiers in plant science

|June 5, 2024

概括

甘露中的基因金字塔增强了俱乐部根的抵抗力. 结合两种俱乐部根抗性 (CR) 基因,CRa和Cr1,显示出对一种新的病原体变体的协同效应,促进了植物防御途径.

科学领域:

植物科学植物科学
分子生物学分子生物学
农业科学农业科学

背景情况:

球根 (Plasmodiophora brassicae) 对加拿大西部的甘生产构成重大威胁.
了解多基因耐药机制,特别是基因相互作用,对于开发耐药的甘油品种至关重要.

研究的目的:

通过比较基因表达特征来研究兰花 (Brassica napus L.) 中球根耐药性的分子机制.
评估金字塔状球根耐药性 (CR) 基因对新型P. brassicae X. 病变型的疗效.

主要方法:

在接种P. brassicae X型病变体后,对三条含有单个 (CRa或Cr1) 或组合 (CRa+Crr1) CR基因的甘油菜系进行转录组分析.
差异表达基因 (DEG) 的功能注释和与植物防御途径相关的基因表达模式的分析.

主要成果:

CR基因Cr1在单基因 (第20系) 和金字塔基因 (第15系) 两种基因系中都对X型病变产生了部分耐药性.
与单一抗性相比,金字塔抗性 (第15行) 显示出与防御相关的DEGs的更高调节,包括那些参与病原体相关的分子模式触发和效应器触发免疫 (PTI和ETI) 的人,与单一抗性 (第20行) 相比.
由于金字塔线中防御基因的增强的转录水平表明了CRa和Cr1之间的协同效应.

结论:

CR基因Cr1激活PTI和ETI信号通路,有助于对X型病变的部分耐药性.
基因堆叠的CRa和Cr1提供了增强的协同抵抗力,以俱乐部根在甘露,超过单个耐药性基因的有效性.

关键词:

布拉斯卡纳普斯 (Brassica napus) 是一种植物.这就是PAMPS.在 PTI 和 ETI 中,有 PTI 和 ETI.这种生物是Plasmodiophora brassicae.在RNA-seqqq.病态型病态型病态型电阻的金字塔结构.

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