相关实验视频

Updated: Jun 24, 2025

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers

Published on: March 3, 2023

从IMMUNEPOTENTCRP分离出来的外体细胞,一种血源,以加速糖尿病伤口愈合

Paola Leonor García Coronado¹, Moisés Armides Franco Molina¹, Diana Ginette Zárate Triviño¹

¹Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.

Frontiers in bioengineering and biotechnology

|June 5, 2024

概括

免疫力强的CRP (ICRP) 成分,特别是外体和外体胰岛素,显著加速糖尿病伤口愈合. 这些疗法增强细胞增殖,增强原蛋白的产生,并通过AKT通路减少炎症.

科学领域:

生物医学科学生物医学科学
伤口治愈研究研究伤口治愈研究
糖尿病并发症糖尿病并发症

背景情况:

糖尿病足带来了截肢的重大风险,需要新的治疗策略.
了解IMMUNEPOTENT CRP (ICRP) 和其组件在伤口修复中的作用,对于开发有效的治疗方法至关重要.

研究的目的:

研究IMMUNEPOTENT CRP (ICRP) 和其分片 (颗粒,超剂,外体,外体胰岛素) 在加速糖尿病伤口愈合方面的治疗潜力.
阐明由ICRP组件介导的潜在机制,包括细胞活力,划伤愈合,原蛋白生产和炎症反应.

主要方法:

ICRP组件被分离和特征,与外体载有胰岛素.
在体外研究评估了NIH-3T3,HUVEC和HACAT细胞系中的细胞活力和痕愈合.
活体研究涉及对患有诱导伤口的糖尿病小鼠模型进行局部应用治疗,随后监测伤口关闭,细胞因子水平和组织学分析.

主要成果:

ICRP颗粒和外体在体外增强了细胞活力.
外体和外体胰岛素配方在体外加速了痕愈合,外体胰岛素显示持续释放胰岛素.
在体内,所有ICRP治疗都加速了伤口的关闭,颗粒,外体和外体胰岛素显示出更高的疗效.
在治愈的组织中,ICRP治疗促进了原表达,并观察到AKT通路组件 (p-AKT,p-FOXO) 的酸化.
所有的治疗都有效地降低了炎症性细胞因子的调节.

结论:

免疫力强的CRP成分,特别是外体和外体胰岛素,是加速糖尿病伤口愈合的有希望的治疗剂.
这些成分促进细胞增殖,原合成,并通过AKT信号通路调节炎症.
基于外体的输送系统,特别是胰岛素,提供了持续释放机制,以改善治疗结果.

关键词:

在Akt路径的Akt路径.免疫力强大的CRP 原蛋白表达的表达方式糖尿病的伤口愈合.外基因组是外基因组的组成部分.

更多相关视频

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Published on: August 4, 2017

Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management

Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management

Published on: February 28, 2025

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.