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使用LC-MS-MS测试人类头发中的质子烯

Pascal Kintz^1,2, Alice Ameline¹, Laurie Gheddar¹

¹Laboratoire de toxicologie, Institut de médecine légale, Strasbourg 67000, France.

Journal of analytical toxicology

|June 5, 2024

概括

一种新方法可以在人类头发中检测出一种强大的合成阿片类药物 - - 质子烯. 这一突破可以识别过量用药的病例和活着的个体,解决日益严重的尼塔对公众健康的威胁.

科学领域:

法医毒理学法医毒理学
分析化学分析化学
药理学药理学是指药理学的学科.

背景情况:

尼塔类的合成本齐米达类阿片类药物Protonitazene在20世纪50年代被开发为止痛药,但由于安全问题从未上市.
尽管自2019年以来它作为一种新型精神活性物质的复苏,但在人类头发中检测到质子烯的情况以前没有报道过.

研究的目的:

开发和验证一种特定的分析程序,用于识别人类头发样本中的protonitazene.
建立一种可靠的方法来检测死后和活体中的protonitazene,以解决其日益增长的流行问题.

主要方法:

开发一种液体染色学-双重质谱法 (LC-MS/MS) 方法用于质子的识别.
头发样本在酸盐缓冲体 (pH 9.5) 中与芬太尼-d5作为内部标准进行化,然后用有机溶剂提取.
通过使用HSS C18柱与15分钟梯度化,实现了染色学分离.

主要成果:

经过验证的方法证明了从1到100ppg/mg的线性,检测极限为0.1ppg/mg.
从广泛的其他阿片类药物,芬太尼衍生物和新型合成阿片类药物中没有观察到干扰.
在两个已故个体 (70和>7600 pg/mg) 和两个活着个体 (14和54 pg/mg) 的头发样本中检测到protonitazene.

结论:

开发的LC-MS/MS方法对于检测人类头发中的质子烯具有特异性和灵敏性.
这种方法为涉及尼塔滥用的法医调查提供了至关重要的工具.
调查结果强调,迫切需要监测和解决与尼塔相关的公共卫生风险.

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