通过调节miR-23a-3p/CCL22轴,LINC00472抑制非小细胞肺癌的进展
Sanhu Yang1, Yongshi Liu2, Yan Li3
1Department of Thoracic Surgery, Second Affiliated Hospital of Air Force Military Medical, Xi'an, Shaanxi 710004, China. tough615@21cn.com.
概括
长非编码RNA LINC00472通过通过海绵化miR-23a-3p增加CCL22来抑制非小细胞肺癌 (NSCLC) 的进展,从而减少细胞生长并促进细胞亡.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 长非编码RNA (lncRNA) LINC00472与瘤发展有关.
- 非小细胞肺癌 (NSCLC) 是一个主要的全球健康问题.
- 了解NSCLC进展背后的分子机制对于开发有效疗法至关重要.
研究的目的:
- 研究LINC00472在NSCLC细胞生物功能中的作用.
- 阐明LINC00472在NSCLC中的潜在分子机制.
- 在NSCLC中探索LINC00472,miR-23a-3p和CCL22之间的相互作用.
主要方法:
- 定量实时PCR (RT-qPCR) 用于确定LINC00472,miR-23a-3p和CCL22的mRNA水平.
- 细胞计数套件-8,细胞划痕检测和西斑检测,以评估细胞增殖,迁移和细胞亡.
- 路西法酶记者测试以验证直接结合相互作用.
主要成果:
- 在NSCLC组织和细胞中发现LINC00472和CCL22的下调,而miR-23a-3p的上调.
- 过度表达LINC00472抑制了NSCLC细胞的增殖和迁移,同时促进了细胞亡.
- LINC00472通过海绵化miR-23a-3p增加了CCL22的表达,而miR-23a-3p模仿剂逆转了这一效应.
- 击败CCL22抵消了LINC00472对NSCLC细胞存活的抑制作用.
结论:
- 在NSCLC中,LINC00472作为瘤抑制剂起作用.
- 通过miR-23a-3p的海绵化,LINC00472通过上调CCL22表达来发挥其瘤抑制作用.
- 针对LINC00472/miR-23a-3p/CCL22轴可能代表NSCLC的潜在治疗策略.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:12Loss of Tumor Suppressor Gene Functions
4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K