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三种用于组织标记墨水的染料用于活检和其他小型标本
Zhenkun Zhu1,2, Quan Li2, Chunxiang Xiang1
1Department of Pathology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, China.
概括
组织学样本标记染料提高了小型活检的可见性. 直接红色227,直接蓝色80和直接紫色66是有效的,不会干扰诊断染色方法.
科学领域:
- 组织病理学 组织病理学
- 手术病理学手术病理学
- 活检分析 活检分析
背景情况:
- 在组织学处理过程中丢失的小活检可以阻碍病理诊断.
- 商用标记染料通常含有专有,未公开的成分.
- 需要具有已知的化学成分的染料,以提高小样本的可见性.
研究的目的:
- 识别具有明确化学成分的合适染料,以提高小样本的可见性.
- 评估用于标记样品的选定直接染料的疗效和诊断兼容性.
主要方法:
- 来自各种器官 (胃,肺,鼻,小肠,哨兵淋巴结) 的样本进行了处理.
- 标记样本使用红色D-FR (直接红色227),蓝色2RL (直接蓝色80) 和紫色D-5BL (直接紫色66).
- 临床病理学家评估了染料的性能和对血素和乙素 (HE) 染色和免疫组织化学的干扰.
主要成果:
- 直接红色227,直接蓝色80和直接紫色66显著提高了小组织样本的可见性.
- 没有一个测试的染料干扰了常规的HE染色.
- 没有观察到与免疫组织化学染色程序的干扰.
结论:
- 直接红色227,直接蓝色80和直接紫色66对于标记小小的活检是有效和安全的.
- 这些染料为改善组织学工作流程中的样本识别提供了可靠的解决方案.
- 这些染料的已知化学成分使得它们比专有替代品更可取.
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