Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

25.5K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Engineering Rapamycin-Induced Dimerization for Control of Gene Expression in Plants.

ACS synthetic biology·2026
Same author

Toward a monocot SynBio toolkit: assessing regulatory element performance and eudicot compatibility.

Plant cell reports·2025
Same author

Increased root growth and seed yield in transgenic soybean overexpressing NAC genes GmNAC19 and GmGRAB1.

Plant cell reports·2025
Same author

Engineering Encapsidated TRV1 as a Complete VIGS Platform.

ACS synthetic biology·2025
Same author

The Potential for Trypsin Inhibitor Expression in Leaves to Convey Herbivory Deterrence in Soybean.

Plants (Basel, Switzerland)·2025
Same author

Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).

Plant cell reports·2024
Same journal

Identification of the R2R3-MYB transcription factor family in goji and LbaR2R3-MYB3 mediated wax biosynthesis enhancing powdery mildew resistance in tobacco.

Plant cell reports·2026
Same journal

Diverse molecular mechanisms of in vivo haploid induction in plants: from fertilization interference to genome programming.

Plant cell reports·2026
Same journal

PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.

Plant cell reports·2026
Same journal

Larix gmelinii UGPase enhances vegetative growth in transgenic poplar through an HYH-mediated positive feedback loop.

Plant cell reports·2026
Same journal

Integrative transcriptional regulatory networks governing cereal root responses to heavy metals and drought.

Plant cell reports·2026
Same journal

Functional characterization of MhbZIP62 in regulating drought tolerance in apple.

Plant cell reports·2026
查看所有相关文章

相关实验视频

Updated: Jun 24, 2025

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

49.2K

强大的大豆叶子农业透.

Bryce N Trull1,2, Mst Shamira Sultana1, Alexander C Pfotenhauer2

  • 1Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.

Plant cell reports
|June 5, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了一种强大的方法,用于使用农业透在大豆叶中的过渡基因表达. 这种技术优化了植物基因型和叶子年龄等因素,以在这个重要的作物中成功传递基因.

关键词:
农业透 农业透在 Betalain Betalain 的位置.红宝石 (Ruby) 是一种红宝石.记者基因 记者基因豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆暂时的基因表达.

更多相关视频

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

20.4K
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
23:21

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Published on: April 19, 2014

41.5K

相关实验视频

Last Updated: Jun 24, 2025

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Published on: July 23, 2013

49.2K
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

20.4K
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
23:21

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Published on: April 19, 2014

41.5K

科学领域:

  • 植物生物技术 植物生物技术
  • 分子生物学分子生物学
  • 农作物科学 农作物科学

背景情况:

  • 农业细菌介导的农业透是一种关键的短暂测试,用于像尼科蒂亚纳 (Nicotiana benthamiana) 这样的二鱼物种.
  • 在作物物种中直接测试基因结构是农业生物技术的理想选择.
  • 大豆 (Glycine max) 是一个具有全球意义的二角作物,需要高效的基因表达工具.

研究的目的:

  • 开发一种强大的农业透方法,用于大豆叶中的短暂基因表达.
  • 确定影响甘氨酸max.农业透成功的关键参数.
  • 为大豆生物技术和合成生物学提供一个新的研究工具.

主要方法:

  • 优化Agrobacterium培养条件和表达载体的优化.
  • 使用Silwet L-77表面活性剂和真空透 (5 分钟在 -85 kPa).
  • 在VC或V1生长阶段选择完全扩张的叶子,然后进行黑暗的化.

主要成果:

  • 在大豆叶中成功地表达了高水平的GUS,光蛋白和RUBY记者 (betalain).
  • 确定大豆农业透成功的关键因素:基因型,表面活性剂,发育阶段和Agrobacterium条件.
  • 在两个大豆品种 (V17-0799DT和TN16-5004) 中证明该方法的有效性.

结论:

  • 已经建立了一个可靠的农业透协议,用于大豆叶的短暂基因表达.
  • 植物基因型,叶子年龄,表面活性剂和Agrobacterium参数对于成功的农业透至关重要.
  • 叶子发育阶段被推测为优化作物物种中的农业透的最关键因素.