相关实验视频

Updated: Jun 24, 2025

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Investigating Target Gene Function in a CD40 Agonistic Antibody-induced Colitis Model using CRISPR/Cas9-based Technologies

Published on: June 2, 2021

一种基于EGFR信号的双对应的aptamer基DNA激动剂有效地减轻了活体性结肠炎

Yulin Cong¹, Kun Liu¹, Zihong Huang¹

¹School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, Guangdong 518107, P. R. China.

ACS chemical biology

|June 5, 2024

概括

一种新型的DNA分子Dimer-YL通过诱导稳定的二分化,有效地激活表皮生长因子受体 (EGFR). 这种DNA激动剂通过促进细胞修复和减少炎症,显示出治疗性结肠炎的前途.

科学领域:

生物化学生物化学
分子生物学分子生物学
胃肠病学胃肠病学

背景情况:

表皮生长因子 (EGF) 通过激活表皮生长因子受体 (EGFR) 信号传导,显示出对性疾病的治疗潜力.
目前的EGF重组蛋白面临的局限性包括稳定性差和修改困难,阻碍了临床应用.
对于治疗性结肠炎的新型EGFR激动剂有着至关重要的需求.

研究的目的:

开发和表征一种基于DNA的新型EGFR激活激素.
评估这种DNA激动剂在促进与肠道修复相关的细胞行为方面的有效性.
评估DNA激动剂在性结肠炎体内模型中的治疗潜力.

主要方法:

设计和合成一种双价亚体DNA分子 (Dimer-YL) 来诱导EGFR二分化.
在体外评估Dimer-YL促进细胞增殖,迁移和修复细胞间紧密结的能力.
在活体中评估Dimer-YL在硫酸 (DSS) 诱导的性结肠炎小鼠模型中的治疗效果.

主要成果:

Dimer-YL成功诱导了稳定的EGFR二分化,重复了EGF促进的细胞行为,如增殖和迁移.
该DNA激动剂在修复受损的细胞间紧密结节方面表现出有效性.
在DSS诱导的性结肠炎模型中,Dimer-YL显著减轻了症状和病理.

结论:

Dimer-YL代表了一种创新的DNA分子,能够通过稳定的受体二分化有效激活EGFR.
这种DNA激动剂为治疗性结肠炎的基于蛋白质的疗法提供了一个有希望的替代方案.
这些发现突显了Dimer-YL作为EGFR介导疾病治疗剂的潜力.

更多相关视频

Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

Published on: January 29, 2019

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

相关概念视频

Drugs for Treatment of Ulcerative Colitis in IBD

Drugs for Treatment of Ulcerative Colitis in IBD

Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...